Kinesin Family Member 11 (KIF11) (AA 324-532) antibody

Details for Product No. ABIN968406
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Antigen
Synonyms F2H17.19, F2H17_19, DDBDRAFT_0189194, DDBDRAFT_0201556, DDB_0189194, DDB_0201556, EG5, HKSP, KNSL1, MCLMR, TRIP5, Eg5, Kif8, Kifl1, Knsl1
Epitope
AA 324-532
(17), (11), (8), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(69), (22), (22), (12), (12), (12), (11), (2), (1)
Host
Mouse
(58), (10), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(48), (24), (18), (16), (15), (13), (12), (10), (5)
Pubmed 2 references available
Quantity 50 μg
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Catalog No. ABIN968406
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Immunogen Human Eg5
Clone LEP-02
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Eg5
Background The mitotic spindle apparatus equally distributes duplicated chromosomes to daughter cells. This process is mediated by the dynamic instability of the spindle microtubules and the forces generated by the action of cytoplasmic dynein and kinesin-related motor proteins (KRPs), such as CHO1/MKLP1. These motors hydrolyze ATP as they move progressively along the microtubules. They organize the microtubules into bipolar spindles and may also serve as bridges between microtubules and chromosome kinetochores or centrosomes. Eg5, identified in Xenopus laevis, is a plus-end directed KRP, associated with the mitotic spindle. Vertebrate Eg5 is a member of the KRP subfamily bimC. These family members share a high degree of sequence homology (>80% within the N-terminal motor domain) and may execute similiar functions. During mitosis, Eg5 is specifically phosphorylated at Thr-297, an evolutionarily conserved cdc2 phosphorylation site, by p34[cdc2]/cyclin B. Inhibition of phosphorylation blocks the interaction of Eg5 with centrosomes. Thus, Eg5 is a mitotic motor protein that regulates spindle formation in a phosphorylation-dependent manner. This antibody is routinely tested by western blot analysis.
Molecular Weight 120 kDa
Research Area Chromatin and Nuclear Signaling, Cell Cycle, Cytoskeleton
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Kinesin Family Member 11 (KIF11) (AA 324-532) antibody Immunofluorescence staining of A431 cells.
anti-Kinesin Family Member 11 (KIF11) (AA 324-532) antibody (2) Western blot analysis of Eg5 on a human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- human Eg5 antibody.
Product cited in: Blangy, Lane, dHérin et al.: "Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo." in: Cell, Vol. 83, Issue 7, pp. 1159-69, 1996 (PubMed).

Ferhat, Cook, Chauviere et al.: "Expression of the mitotic motor protein Eg5 in postmitotic neurons: implications for neuronal development." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 19, pp. 7822-35, 1998 (PubMed).

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