Baculoviral IAP Repeat-Containing 6 (BIRC6) (AA 372-571) antibody

Details for Product No. ABIN968408
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Synonyms BIRC6, bruce, apollon, APOLLON, BRUCE, A430032G04Rik, A430040A19Rik, AA501170, Bruce, D630005A10Rik, mKIAA1289
AA 372-571
(25), (13), (2), (1), (1), (1), (1), (1)
Mouse (Murine)
(57), (5), (4), (3), (2), (2), (2), (2), (1), (1)
(32), (25), (1)
Clonality (Clone)
Monoclonal ()
(4), (4), (4), (4), (4), (4)
Western Blotting (WB), Immunofluorescence (IF)
(42), (19), (10), (7), (5), (4), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
Shipping to United States (Change)
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Catalog No. ABIN968408
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Immunogen Mouse BRUCE
Clone 4
Isotype IgG1
Cross-Reactivity Human, Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name BRUCE
Background Selective proteolysis is essential for the modulation of key cellular processes such as cell cycle progression. However, unlike other post-translational events, proteolysis is irreversible and therefore must occur in unidirectional cellular pathways. In eukaryotes, proteolysis is mediated primarily by the ubiquitin pathway. This pathway designates proteins for degradation by the proteasome, a multicatalytic protease complex. The ubiquitin pathway is a multistep system that tags proteins for degradation via the attachment of ubiquitin molecules to the target. This attachment is mediated by the ubiquitin activating/conjugating enzymes E1, E2, E3, and BRUCE. BRUCE (BIR Repeat containing Ubiquitin-Conjugating Enzyme) is a novel enzyme that associates with the Golgi and the vesicular system. It contains a UBC (ubiquitin conjugating enzyme) domain, which is essential for catalysis, and a BIR (baculovirus inhibitor of apoptosis repeat) motif. BIR motifs are also found within inhibitor of apoptosis proteins (IAP) and are critical for anti-apoptotic activity. Therefore, BRUCE may function to both mediate ubiquitin-dependent proteolysis and contribute to anti-apoptotic cellular pathways.
Synonyms: BIR Repeat containing Ubiquitin-Conjugating Enzyme
Molecular Weight 528 kDa
Application Notes Clone 4/BRUCE has also been shown to work well for Western blot application on Hela lysate (ABIN968535)

Related Products: ABIN968552, ABIN967389, ABIN968535

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Baculoviral IAP Repeat-Containing 6 (BIRC6) (AA 372-571) antibody Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2.2).
anti-Baculoviral IAP Repeat-Containing 6 (BIRC6) (AA 372-571) antibody (2) Western blot analysis of BRUCE on a SW-13 cell lysate (Human adrenal gland carcinoma, ATCC CCL-105). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-BRUCE antibody.
Product cited in: Hauser, Bardroff, Pyrowolakis et al.: "A giant ubiquitin-conjugating enzyme related to IAP apoptosis inhibitors." in: The Journal of cell biology, Vol. 141, Issue 6, pp. 1415-22, 1998 (PubMed).

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