Baculoviral IAP Repeat-Containing 6 (BIRC6) (AA 372-571) antibody
|Synonyms||Bruce, AA501170, mKIAA1289, A430032G04Rik, A430040A19Rik, D630005A10Rik, BRUCE, APOLLON, FLJ13726, FLJ13786, KIAA1289, BIRC6, bruce, apollon|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|1 reference available|
|Price||Product not available in this region.|
|Cross-Reactivity||Human, Rat (Rattus), Dog (Canine)|
Selective proteolysis is essential for the modulation of key cellular processes such as cell cycle progression. However, unlike other post-translational events, proteolysis is irreversible and therefore must occur in unidirectional cellular pathways. In eukaryotes, proteolysis is mediated primarily by the ubiquitin pathway. This pathway designates proteins for degradation by the proteasome, a multicatalytic protease complex. The ubiquitin pathway is a multistep system that tags proteins for degradation via the attachment of ubiquitin molecules to the target. This attachment is mediated by the ubiquitin activating/conjugating enzymes E1, E2, E3, and BRUCE. BRUCE (BIR Repeat containing Ubiquitin-Conjugating Enzyme) is a novel enzyme that associates with the Golgi and the vesicular system. It contains a UBC (ubiquitin conjugating enzyme) domain, which is essential for catalysis, and a BIR (baculovirus inhibitor of apoptosis repeat) motif. BIR motifs are also found within inhibitor of apoptosis proteins (IAP) and are critical for anti-apoptotic activity. Therefore, BRUCE may function to both mediate ubiquitin-dependent proteolysis and contribute to anti-apoptotic cellular pathways.
Synonyms: BIR Repeat containing Ubiquitin-Conjugating Enzyme
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||528 kDa|
Related Products: ABIN968552, ABIN967389, ABIN968535
|Application Notes||Clone 4/BRUCE has also been shown to work well for Western blot application on Hela lysate (ABIN968535)|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
Hauser, Bardroff, Pyrowolakis et al.: "A giant ubiquitin-conjugating enzyme related to IAP apoptosis inhibitors." in: The Journal of cell biology, Vol. 141, Issue 6, pp. 1415-22, 1998 (PubMed).
|Hosts||Rabbit (27), Goat (4)|
|Reactivities||Human (28), Cow (Bovine) (18), Horse (Equine) (18), Mouse (Murine) (18), Pig (Porcine) (18), Rat (Rattus) (18)|
|Applications||Immunofluorescence (IF) (15), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1), Immunoprecipitation (IP) (1), Western Blotting (WB) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (5), AA 37-52 (1), AA 3855-3872 (1), AA 4724-4735 (1), AA 892-907 (1), Internal Region (1)|