BIRC6 antibody (AA 372-571)

Catalog No. ABIN968409

Product Details anti-BIRC6 Antibody

Target: BIRC6 (Baculoviral IAP Repeat-Containing 6 (BIRC6))

Binding Specificity: AA 372-571

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This BIRC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Human, Rat (Rattus), Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse BRUCE aa. 372-571

Clone: 4-BRUCE

Isotype: IgG1

Application Details

Application Notes: Clone 4/BRUCE has also been shown to work well for Western blot application on Hela lysate (ABIN968535)

Comment:

Related Products: ABIN968552, ABIN967389, ABIN968535

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-BIRC6 antibody (ABIN968409)

Hauser, Bardroff, Pyrowolakis, Jentsch: "A giant ubiquitin-conjugating enzyme related to IAP apoptosis inhibitors." in: The Journal of cell biology, Vol. 141, Issue 6, pp. 1415-22, (1998) (PubMed).

Target Details for BIRC6

Target: BIRC6 (Baculoviral IAP Repeat-Containing 6 (BIRC6))

Alternative Name: BRUCE (BIRC6 Products)

Synonyms: APOLLON antibody, BRUCE antibody, bruce antibody, apollon antibody, BIRC6 antibody, A430032G04Rik antibody, A430040A19Rik antibody, AA501170 antibody, Bruce antibody, D630005A10Rik antibody, mKIAA1289 antibody, baculoviral IAP repeat containing 6 antibody, baculoviral IAP repeat-containing 6 antibody, BIRC6 antibody, Birc6 antibody, birc6 antibody

Background: Selective proteolysis is essential for the modulation of key cellular processes such as cell cycle progression. However, unlike other post-translational events, proteolysis is irreversible and therefore must occur in unidirectional cellular pathways. In eukaryotes, proteolysis is mediated primarily by the ubiquitin pathway. This pathway designates proteins for degradation by the proteasome, a multicatalytic protease complex. The ubiquitin pathway is a multistep system that tags proteins for degradation via the attachment of ubiquitin molecules to the target. This attachment is mediated by the ubiquitin activating/conjugating enzymes E1, E2, E3, and BRUCE. BRUCE (BIR Repeat containing Ubiquitin-Conjugating Enzyme) is a novel enzyme that associates with the Golgi and the vesicular system. It contains a UBC (ubiquitin conjugating enzyme) domain, which is essential for catalysis, and a BIR (baculovirus inhibitor of apoptosis repeat) motif. BIR motifs are also found within inhibitor of apoptosis proteins (IAP) and are critical for anti-apoptotic activity. Therefore, BRUCE may function to both mediate ubiquitin-dependent proteolysis and contribute to anti-apoptotic cellular pathways.
Synonyms: BIR Repeat containing Ubiquitin-Conjugating Enzyme

Molecular Weight: 528 kDa