Melanoma Cell Adhesion Molecule (MCAM) (AA 104-305) antibody

Antigen: Melanoma Cell Adhesion Molecule (MCAM)

Synonyms: CD146, MUC18, Muc18, CD149, s-endo, AV025631, 1-gicerin, s-gicerin, MCAM, cd146, gicerin, id:ibd3512

Binding Site: AA 104-305

Clonality: Monoclonal (1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968422

Additional Information

Alternative name: MCAM

Immunogen: Human MCAM

Format: Liquid

Isotype: IgG1

Clone: 1

Description: Tumor metastasis is determined by the metastatic capacity of subpopulations of tumor cells. The presence of cell adhesion molecules (CAMs) is a property of tumor cells that is essential for metastasis. MCAM (MUC18 or Mel-CAM) was identified on melanoma cells and is one of several CAMs that belong to the immunoglobulin superfamily. MCAM is an integral membrane glycoprotein that consists of five immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic domain. The presence of several protein kinase recognition motifs in the cytoplasmic domain suggest a role for MCAM in signal transduction. MCAM is capable of both homotypic and heterotypic adhesion between cells, but its heterotypic ligand remains to be identified. It is sparsely expressed on benign melanocytic nevi and thin primary melanomas. However, MCAM expression increases with tumor thickness. It is expressed on 70-80% of advanced primary and metastatic tumors. Additionally, S-Endo-1 antigen (CD146), present on human endothelial cells, is identical to MCAM. Thus, MCAM may play an important role in the development of metastatic tumors and possibly represents a prime target for therapeutic treatment. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 113 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of MCAM on a human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- MCAM antibody.

Immunofluorescent staining of human endothelial cells.

anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 104-305) antibody (Image 3)

Publications

Product: Lehmann, Riethmüller, Johnson: "MUC18, a marker of tumor progression in human melanoma, shows sequence similarity to the neural cell adhesion molecules of the immunoglobulin superfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 24, pp. 9891-5, 1990 (PubMed).

Xie, Luca, Huang et al.: "Expression of MCAM/MUC18 by human melanoma cells leads to increased tumor growth and metastasis." in: Cancer research, Vol. 57, Issue 11, pp. 2295-303, 1997 (PubMed).

Jean, Gershenwald, Huang et al.: "Loss of AP-2 results in up-regulation of MCAM/MUC18 and an increase in tumor growth and metastasis of human melanoma cells." in: The Journal of biological chemistry, Vol. 273, Issue 26, pp. 16501-8, 1998 (PubMed).

Anfosso, Bardin, Francès et al.: "Activation of human endothelial cells via S-endo-1 antigen (CD146) stimulates the tyrosine phosphorylation of focal adhesion kinase p125(FAK)." in: The Journal of biological chemistry, Vol. 273, Issue 41, pp. 26852-6, 1998 (PubMed).