MKK3b (AA 12-114) antibody

Details for Product No. ABIN968425
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Antigen
Epitope
AA 12-114
Reactivity
Human
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN968425
Quantity 50 µg
Price
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Immunogen Human MKK3b
Clone OPG-13
Isotype IgG2a
Cross-Reactivity Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Background Mitogen activated protein (MAP) kinase signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. Three groups of mammalian MAP kinases have been identified: the extracellular signal-regulated kinases (ERK), the c-Jun N-terminal kinases (JNK), and the p38 MAP kinases. The p38 MAP kinases are activated by dual phosphorylation on Thr and Tyr within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP kinase kinases MKK3 and MKK6, which are activated by a MAP kinase kinase kinase (MKKK). MKK3b, an alternatively spliced isoform of MKK3, contains an extra 29 N-terminal amino acids. Like MKK3 and MKK6, MKK3b specifically activates p38 MAPK and is more efficient than MKK3 in mediating downstream signaling events. Although MKK6 expression is limited, MKK3 and MKK3b are expressed in a wide range of cell types. MKK3, MKK3b, and MKK6 function as specific activators of p38 MAPK. However, they differ in their expression patterns and strength of kinase activity and, therefore, may differentially regulate p38 MAPK activation.
Molecular Weight 37 kDa
Comment

Related Products: ABIN967389, ABIN968535

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-MKK3b (AA 12-114) antibody Western blot analysis of MKK3b on HeLa lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of MKK3b.
anti-MKK3b (AA 12-114) antibody (2) anti-MKK3b (AA 12-114) antibody (Image 2)
Product cited in: Moriguchi, Toyoshima, Gotoh et al.: "Purification and identification of a major activator for p38 from osmotically shocked cells. Activation of mitogen-activated protein kinase kinase 6 by osmotic shock, tumor necrosis factor-alpha, and H2O2." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 26981-8, 1996 (PubMed).

Han, Wang, Jiang et al.: "Identification and characterization of a predominant isoform of human MKK3." in: FEBS letters, Vol. 403, Issue 1, pp. 19-22, 1997 (PubMed).

Enslen, Raingeaud, Davis: "Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1741-8, 1998 (PubMed).

Rincón, Enslen, Raingeaud et al.: "Interferon-gamma expression by Th1 effector T cells mediated by the p38 MAP kinase signaling pathway." in: The EMBO journal, Vol. 17, Issue 10, pp. 2817-29, 1998 (PubMed).

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Order hotline:

  • +1 404 474 4654
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