MKK3b (AA 12-114) antibody

Antigen: MKK3b

Binding Site: AA 12-114

Clonality: Monoclonal (30)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN968425

Additional Information

Immunogen: Human MKK3b

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Format: Liquid

Isotype: IgG2a

Clone: 30

Description: Mitogen activated protein (MAP) kinase signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. Three groups of mammalian MAP kinases have been identified: the extracellular signal-regulated kinases (ERK), the c-Jun N-terminal kinases (JNK), and the p38 MAP kinases. The p38 MAP kinases are activated by dual phosphorylation on Thr and Tyr within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP kinase kinases MKK3 and MKK6, which are activated by a MAP kinase kinase kinase (MKKK). MKK3b, an alternatively spliced isoform of MKK3, contains an extra 29 N-terminal amino acids. Like MKK3 and MKK6, MKK3b specifically activates p38 MAPK and is more efficient than MKK3 in mediating downstream signaling events. Although MKK6 expression is limited, MKK3 and MKK3b are expressed in a wide range of cell types. MKK3, MKK3b, and MKK6 function as specific activators of p38 MAPK. However, they differ in their expression patterns and strength of kinase activity and, therefore, may differentially regulate p38 MAPK activation.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 37 kDa

Comments:

Related Products: ABIN967389, ABIN968535

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of MKK3b on HeLa lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of MKK3b.

anti-MKK3b (AA 12-114) antibody (Image 2)

Publications

Product: Moriguchi, Toyoshima, Gotoh et al.: "Purification and identification of a major activator for p38 from osmotically shocked cells. Activation of mitogen-activated protein kinase kinase 6 by osmotic shock, tumor necrosis factor-alpha, and H2O2." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 26981-8, 1996 (PubMed).

Han, Wang, Jiang et al.: "Identification and characterization of a predominant isoform of human MKK3." in: FEBS letters, Vol. 403, Issue 1, pp. 19-22, 1997 (PubMed).

Enslen, Raingeaud, Davis: "Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1741-8, 1998 (PubMed).

Rincón, Enslen, Raingeaud et al.: "Interferon-gamma expression by Th1 effector T cells mediated by the p38 MAP kinase signaling pathway." in: The EMBO journal, Vol. 17, Issue 10, pp. 2817-29, 1998 (PubMed).