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Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1) (AA 46-58) antibody

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Antigen
Synonyms ubch6, UBCH6, UbcM3, Ubce5, ubcM2
Epitope
AA 46-58
(2), (2), (1)
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(16), (14), (7), (3), (1), (1)
Host
Mouse
(20)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(11), (10), (3), (1)
Pubmed 3 references available
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Quantity 50 μg
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Immunogen Human UbcH6 aa. 46-58
Clone 42-UbcH6
Isotype IgG1
Cross-Reactivity Dog (Canine), Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name UbcH6 (UBE2E1 Antibody Abstract)
Background Maintenance of cellular function requires timely and selective degradation of key regulatory proteins. For example, progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins via the ubiquitin pathway. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an epsilon-NH2-Lys in a target protein. Ubiquitin-conjugated proteins are recognized and degraded by the 26S proteasome. Ubiquitination requires ubiquitin-activating enzyme E1, ubiquitin conjugating enzymes E2, and ubiquitin ligases E3. The direction of ubiquitin transfer is from E1 to E2 and from E2 to E3. E6-AP is a human E3 which, in complex with the E6 protein of human papillomaviruses, induces the ubiquitination and degradation of p53. UbcH6 is a human homolog of the S. cerevisiae ubiquitin-conjugating enzyme (Ubc), UBC4, which interacts with E6-AP. UbcH6 also interacts with E6-AP. However, it has little effect on E6-AP-dependent ubiquitination. The precise physiological function of UbcH6 remains to be determined.
Molecular Weight 21 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1) (AA 46-58) antibody (ABIN968426) Western blot analysis of UbcH6 on human endothelial lysate. Lane 1: 1:1000, lane 2: 1...
Image no. 2 for anti-Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1) (AA 46-58) antibody (ABIN968426) Human Endothelial
Image no. 3 for anti-Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1) (AA 46-58) antibody (ABIN968426) anti-Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1) (AA 46-58) antibody (Image 3)
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1) (AA 46-58) antibody (ABIN968426) anti-Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1) (AA 46-58) antibody (Image 4)
Product cited in: Hagglund, Van Sant, Lopez et al.: "Herpes simplex virus 1-infected cell protein 0 contains two E3 ubiquitin ligase sites specific for different E2 ubiquitin-conjugating enzymes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 2, pp. 631-6, 2002 (PubMed).

Nuber, Schwarz, Kaiser et al.: "Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5." in: The Journal of biological chemistry, Vol. 271, Issue 5, pp. 2795-800, 1996 (PubMed).

Scheffner, Huibregtse, Howley: "Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 19, pp. 8797-801, 1994 (PubMed).

Catalog No. ABIN968426
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