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Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (AA 176-289) antibody

Details for Product No. ABIN968431, Supplier: Login to see
Antigen
  • GR
  • gcr
  • grl
  • xGR
  • gccr
  • gr
  • zgc:113038
  • NR3C1
  • GCL
  • LOC100302444
  • Gcr
  • Grl
  • Grl-1
  • Grl1
  • GRL
  • GCCR
  • GCR
  • NO
  • GR-A
  • nr3c1
Alternatives
anti-Human Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) antibody for Western Blotting
Epitope
AA 176-289
37
23
18
17
16
15
14
14
13
11
10
7
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
249
86
80
4
2
2
2
2
Host
Mouse
204
58
6
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
21
16
9
7
6
6
3
3
3
3
3
3
3
3
3
Application
BioImaging (BI), Immunofluorescence (IF), Western Blotting (WB)
191
107
78
46
44
33
32
20
18
8
3
2
2
1
1
Supplier
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Immunogen Human Glucocorticoid Receptor alpha aa. 176-289
Clone 41-Glucocorticoid Receptor
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Glucocorticoid Receptor (NR3C1 Antibody Abstract)
Background Steroid hormone receptors are hormone activated transcriptional regulators that influence genes required for embryonic development and adult homeostasis. One member of the steroid hormone family is the glucocorticoid receptor. It contains AF1 and AF2 transactivation domains, a DNA binding domain, and ligand binding domain. Ligand bound glucocorticoid receptors dimerize at specific palindromic sequences called glucocorticoid response elements (GREs) in the cis-regulatory region of target genes. Both AF1 and AF2 may be important for initiation or regulation of transcription by interacting with components of the initiation complex or other intermediary factors. In addition to transactivation, glucocorticoid receptors may also regulate transcription through transrepression of target genes. Although mechanisms of transrepression are not completely understood, DNA binding alone, DNA binding plus interaction with other transcription factors, or protein-protein interaction without DNA binding are mechanisms that have been implicated. Thus, glucocorticoid receptors function in the regulation of specific genes that are essential for human development and homeostasis. This antibody is routinely tested by western blot analysis.
Molecular Weight 94 kDa
Research Area Chromatin and Nuclear Signaling, Hormones, Receptors
Pathways Nuclear Receptor Transcription Pathway
Comment

Related Products: ABIN967389, ABIN968535

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (AA 176-289) antibody (ABIN968431) Western blot analysis of glucocorticoid receptor on HeLa cell lysate (right). Lane 1:...
Immunofluorescence (IF) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (AA 176-289) antibody (ABIN968431) Immunofluorescent staining of HCT-8 cells.
 image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (AA 176-289) antibody (ABIN968431) anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (AA 176-289) antibody (Image 3)
Immunofluorescence (IF) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (AA 176-289) antibody (ABIN968431) anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (AA 176-289) antibody (Image 4)
Western Blotting (WB) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (AA 176-289) antibody (ABIN968431) anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (AA 176-289) antibody (Image 5)
Product cited in: Reichardt, Kaestner, Tuckermann et al.: "DNA binding of the glucocorticoid receptor is not essential for survival." in: Cell, Vol. 93, Issue 4, pp. 531-41, 1998 (PubMed).

Beato, Herrlich, Schütz: "Steroid hormone receptors: many actors in search of a plot." in: Cell, Vol. 83, Issue 6, pp. 851-7, 1996 (PubMed).

Hollenberg, Weinberger, Ong et al.: "Primary structure and expression of a functional human glucocorticoid receptor cDNA." in: Nature, Vol. 318, Issue 6047, pp. 635-41, 1986 (PubMed).