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Integrin beta 4 antibody (ITGB4) (AA 1612-1821)

Details for Product anti-ITGB4 Antibody No. ABIN968434, Supplier: Log in to see
Antigen
  • AA407042
  • C230078O20
  • CD104
Epitope
AA 1612-1821
13
13
13
13
9
5
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
182
131
78
2
2
Host
Mouse
113
77
66
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Integrin beta 4 antibody is un-conjugated
28
16
16
7
5
5
4
4
4
4
4
4
4
1
Application
Immunofluorescence (IF), Western Blotting (WB)
138
125
63
55
48
45
38
28
15
9
5
5
3
2
2
2
1
1
1
1
Supplier
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Immunogen Human Integrin beta4 aa. 1612-1821
Clone 7-CD104
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD104 (ITGB4 Antibody Abstract)
Background Cell adhesion to extracellular matrix components or to cell surface proteins, especially those expressed by leukocytes and endothelial cells, is mediated by integrins. Integrins contain noncovalently associated alpha and beta subunits. At least 17 alpha and 8 beta subunits have been identified and these proteins can heterodimerize to form 22 different receptors. The alpha6beta4 integrin is a receptor for various laminins and binds with the highest affinity to laminins 4 and 5. It exhibits elevated expression in the basal cell layer of stratified epithelia, in Schwann cells at the onset of myelination, and in CD4-CD8- pre-T lymphocytes entering the thymus. In addition, alpha6beta4 expression is increased in squamous carcinomas where it promotes invasion through a targeting of PI3 kinase activity. The majority of beta4 comprises a cytoplasmic domain with unique signaling properties. The C-terminal portion of this domain contains two pairs of type III fibronectin-like motifs (FNIII) and a tyrosine activation motif (TAM). Additional domains in the cytoplasmic tail bind Shc and activate the MAPK pathway. Thus, integrin beta4 is an integrin subunit that is important for cell survival, growth, and differentiation.
Synonyms: Integrin beta4
Molecular Weight 200 kDa
Research Area CD Antigens, Surface Receptors of Immune Cells
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 4 antibody (ITGB4) (AA 1612-1821) (ABIN968434) Western blot analysis of CD104 (Integrin beta4) on a A431 cell lysate (Human epitheli...
Immunofluorescence (IF) image for anti-Integrin beta 4 antibody (ITGB4) (AA 1612-1821) (ABIN968434) Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).
 image for anti-Integrin beta 4 antibody (ITGB4) (AA 1612-1821) (ABIN968434) anti-Integrin beta 4 (ITGB4) (AA 1612-1821) antibody (Image 3)
Western Blotting (WB) image for anti-Integrin beta 4 antibody (ITGB4) (AA 1612-1821) (ABIN968434) anti-Integrin beta 4 (ITGB4) (AA 1612-1821) antibody (Image 4)
Product cited in: Shaw, Rabinovitz, Wang et al.: "Activation of phosphoinositide 3-OH kinase by the alpha6beta4 integrin promotes carcinoma invasion." in: Cell, Vol. 91, Issue 7, pp. 949-60, 1998 (PubMed).

Mainiero, Pepe, Wary et al.: "Signal transduction by the alpha 6 beta 4 integrin: distinct beta 4 subunit sites mediate recruitment of Shc/Grb2 and association with the cytoskeleton of hemidesmosomes." in: The EMBO journal, Vol. 14, Issue 18, pp. 4470-81, 1995 (PubMed).