Neuropilin 2 (NRP2) (AA 610-721) antibody
|Synonyms||NP2, NPN2, PRO2714, MGC126574, VEGF165R2|
Alternatives Western Blotting (WB)
|4 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
The precise navigation of axons to their targets in the developing nervous system is essential for establishment of neuronal connectivity. This is achieved through the recognition of attractant and repellent signals delivered to neurons by the netrins and semaphorins, which interact with receptors on axons and growth cones. The semaphorins are a large family of secreted and transmembrane glycoproteins that function as chemorepellents of developing neurons. Neuropilin-1 (NP-1) and neuropilin-2 (NP-2) (44% amino acid identity) are receptors for various members of the secreted semaphorin family. While NP-1 binds to class III semaphorins (Sema III, Sema E, and Sema IV), NP-2 is a high affinity receptor for Sema E and Sema IV, but not Sema III. Although they interact with distinct but overlapping subsets of semaphorins, NP-1 and NP-2 exhibit unique expression patterns during nervous system development. Moreover, NP-1 and NP-2 can form homooligomers which can, in turn, form heterooligomers. Thus, their interactions and differential patterns of expression implicate NP-1 and NP-2 in the coordination of axon guidance during neurodevelopment.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||104 kDa|
Related Products: ABIN968545, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
|Western blot analysis of Neuropilin-2 on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-rat Neuropilin-2 antibody. anti-Neuropilin 2 (NRP2) (AA 610-721) antibody (Image 2)|
Kolodkin, Levengood, Rowe et al.: "Neuropilin is a semaphorin III receptor." in: Cell, Vol. 90, Issue 4, pp. 753-62, 1997 (PubMed).
Chen, Chédotal, He et al.: "Neuropilin-2, a novel member of the neuropilin family, is a high affinity receptor for the semaphorins Sema E and Sema IV but not Sema III." in: Neuron, Vol. 19, Issue 3, pp. 547-59, 1997 (PubMed).
Martin, Witte, Wong: "The synthesis and enzymatic incorporation of sialic acid derivatives for use as tools to study the structure, activity, and inhibition of glycoproteins and other glycoconjugates." in: Bioorganic & medicinal chemistry, Vol. 6, Issue 8, pp. 1283-92, 1999 (PubMed).
Giger, Urquhart, Gillespie et al.: "Neuropilin-2 is a receptor for semaphorin IV: insight into the structural basis of receptor function and specificity." in: Neuron, Vol. 21, Issue 5, pp. 1079-92, 1999 (PubMed).
|Hosts||Rabbit (28), Chicken (1), Mouse (1)|
|Reactivities||Human (29), Rat (Rattus) (23), Mouse (Murine) (22), Chicken (19), Cow (Bovine) (19), Pig (Porcine) (18), Cat (Feline) (1), Dog (Canine) (1), Hamster (1)|
|Applications||Immunofluorescence (IF) (16), Flow Cytometry (FACS) (15), Western Blotting (WB) (15), ELISA (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (IHC) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term,Lys813 (2), AA 432-547 (1), N-Term (1)|