Neuropilin 2 (NRP2) (AA 610-721) antibody

Antigen: Neuropilin 2 (NRP2)

Synonyms: NP2, NPN2, PRO2714, MGC126574, VEGF165R2

Binding Site: AA 610-721

Clonality: Monoclonal (54)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968443

Additional Information

Alternative name: Neuropilin-2

Immunogen: Rat Neuropilin-2

Format: Liquid

Isotype: IgG1

Clone: 54

Description: The precise navigation of axons to their targets in the developing nervous system is essential for establishment of neuronal connectivity. This is achieved through the recognition of attractant and repellent signals delivered to neurons by the netrins and semaphorins, which interact with receptors on axons and growth cones. The semaphorins are a large family of secreted and transmembrane glycoproteins that function as chemorepellents of developing neurons. Neuropilin-1 (NP-1) and neuropilin-2 (NP-2) (44% amino acid identity) are receptors for various members of the secreted semaphorin family. While NP-1 binds to class III semaphorins (Sema III, Sema E, and Sema IV), NP-2 is a high affinity receptor for Sema E and Sema IV, but not Sema III. Although they interact with distinct but overlapping subsets of semaphorins, NP-1 and NP-2 exhibit unique expression patterns during nervous system development. Moreover, NP-1 and NP-2 can form homooligomers which can, in turn, form heterooligomers. Thus, their interactions and differential patterns of expression implicate NP-1 and NP-2 in the coordination of axon guidance during neurodevelopment.
Synonyms: NP-2

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 104 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Neuropilin-2 on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-rat Neuropilin-2 antibody.

anti-Neuropilin 2 (NRP2) (AA 610-721) antibody (Image 2)

Publications

Product: Kolodkin, Levengood, Rowe et al.: "Neuropilin is a semaphorin III receptor." in: Cell, Vol. 90, Issue 4, pp. 753-62, 1997 (PubMed).

Chen, Chédotal, He et al.: "Neuropilin-2, a novel member of the neuropilin family, is a high affinity receptor for the semaphorins Sema E and Sema IV but not Sema III." in: Neuron, Vol. 19, Issue 3, pp. 547-59, 1997 (PubMed).

Martin, Witte, Wong: "The synthesis and enzymatic incorporation of sialic acid derivatives for use as tools to study the structure, activity, and inhibition of glycoproteins and other glycoconjugates." in: Bioorganic & medicinal chemistry, Vol. 6, Issue 8, pp. 1283-92, 1999 (PubMed).

Giger, Urquhart, Gillespie et al.: "Neuropilin-2 is a receptor for semaphorin IV: insight into the structural basis of receptor function and specificity." in: Neuron, Vol. 21, Issue 5, pp. 1079-92, 1999 (PubMed).