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Flap Structure-Specific Endonuclease 1 (FEN1) (AA 252-371) antibody

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Synonyms FEN-1, FEN1, AW538437, MF1, RAD2, cb879, fen-1, fen1, mf1, rad2, xFEN1a
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AA 252-371
(8), (8), (8), (7), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(105), (40), (37), (3), (2), (1), (1), (1), (1), (1)
(77), (29)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
BioImaging (BI), Western Blotting (WB)
(84), (23), (19), (19), (14), (12), (11), (10), (3), (3), (1), (1)
Pubmed 5 references available
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Quantity 50 μg
Shipping to United States ( )
Immunogen Human FEN-1 aa. 252-371
Clone 21-FEN
Isotype IgG1
Cross-Reactivity Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name FEN-1 (FEN1 Antibody Abstract)
Background The fidelity of DNA replication, recombination, and repair is essential for genome stability. Proteins that have exonuclease and endonuclease enzymatic activity are critical for accurate replication, recombination, and repair of DNA sequences. FEN-1 ( five' exonuclease-1 or flap endonuclease-1) is an exo- and endonuclease found in yeast, mouse, and human. The 5'-3' exonuclease activity of FEN-1 involves only double stranded DNA and is important for processing Okazaki fragments during lagging strand DNA synthesis. In addition, FEN-1 is a member of the RAD2 family of repair nucleases and may participate in DNA repair. Its endonuclease activity is specific for 5' overhanging flaps, which are intermediates in the repair of DNA double strand breaks. Human and mouse FEN-1 are highly conserved with 95% identity at the amino acid level. FEN-1 expression is highest during entry into the mitotic cell cycle and lowest in differentiated cells. Thus, FEN-1 is an exo- and endonuclease that is thought to be essential for maintaining DNA integrity during replication, as well as after damage by alkylating agents or UV.
Molecular Weight 50 kDa
Pathways Telomere Maintenance, DNA Damage Repair
Application Notes Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

Related Products: ABIN967389, ABIN968536

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (AA 252-371) antibody (ABIN968459) Western blot analysis of FEN-1 on a human endothelial lysate. Lane 1: 1:250, lane 2: ...
Immunofluorescence (IF) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (AA 252-371) antibody (ABIN968459) Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96 well...
 image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (AA 252-371) antibody (ABIN968459) anti-Flap Structure-Specific Endonuclease 1 (FEN1) (AA 252-371) antibody (Image 3)
Product cited in: Yoon, Swiderski, Kaplan et al.: "Processing of UV damage in vitro by FEN-1 proteins as part of an alternative DNA excision repair pathway." in: Biochemistry, Vol. 38, Issue 15, pp. 4809-17, 1999 (PubMed).

Kim: "Down-regulation of human FEN-1 gene expression during differentiation of promyelocytic leukemia cells." in: Experimental & molecular medicine, Vol. 30, Issue 4, pp. 252-6, 1999 (PubMed).

Hosfield, Mol, Shen et al.: "Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity." in: Cell, Vol. 95, Issue 1, pp. 135-46, 1998 (PubMed).

Harrington, Lieber: "The characterization of a mammalian DNA structure-specific endonuclease." in: The EMBO journal, Vol. 13, Issue 5, pp. 1235-46, 1994 (PubMed).

Hiraoka, Harrington, Gerhard et al.: "Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human." in: Genomics, Vol. 25, Issue 1, pp. 220-5, 1995 (PubMed).

Catalog No. ABIN968459
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