STXBP5 antibody (AA 910-1105)

Catalog No. ABIN968460

Product Details anti-STXBP5 Antibody

Target: STXBP5 (Syntaxin Binding Protein 5 (Tomosyn) (STXBP5))

Binding Specificity: AA 910-1105

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This STXBP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

Cross-Reactivity: Mouse (Murine), Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Tomosyn aa. 910-1105

Clone: 15-Tomosyn

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-STXBP5 antibody (ABIN968460)

Yokoyama, Shirataki, Sakisaka, Takai: "Three splicing variants of tomosyn and identification of their syntaxin-binding region." in: Biochemical and biophysical research communications, Vol. 256, Issue 1, pp. 218-22, (1999) (PubMed).

Fujita, Shirataki, Sakisaka, Asakura, Ohya, Kotani, Yokoyama, Nishioka, Matsuura, Mizoguchi, Scheller, Takai: "Tomosyn: a syntaxin-1-binding protein that forms a novel complex in the neurotransmitter release process." in: Neuron, Vol. 20, Issue 5, pp. 905-15, (1998) (PubMed).

Target Details for STXBP5

Target: STXBP5 (Syntaxin Binding Protein 5 (Tomosyn) (STXBP5))

Alternative Name: Tomosyn (STXBP5 Products)

Synonyms: LGL3 antibody, LLGL3 antibody, Nbla04300 antibody, 0710001E20Rik antibody, 4930565N16Rik antibody, AW742610 antibody, mKIAA1006 antibody, syntaxin binding protein 5 antibody, syntaxin binding protein 5 (tomosyn) antibody, STXBP5 antibody, Stxbp5 antibody

Background: Neuronal signal transmission and neurotransmitter release from the presynaptic nerve terminal is mediated by the synaptic vesicle cycle. Syntaxin plays a central role during vesicle docking and fusion through interactions with many vesicle components. During docking, vSNAREs (VAMP/synaptobrevin, synaptotagmin) on the synaptic vesicle and tSNAREs (SNAP-25, syntaxin) on the plasma membrane interact to form a 7S complex, which is essential to docking and fusion. Syntaxin associates with munc18/n-sec1 prior to and/or during the formation of the 7S complex. This interaction may inhibit syntaxin binding proteins (VAMP, SNAP-25) that facilitate vesicle docking or fusion. Tomosyn, a syntaxin binding protein, displaces munc18 from syntaxin-1 and forms a novel 10S complex with syntaxin-1, SNAP-25, and synaptogamin. There are two splice variants of tomosyn designated b-tomosyn and s-tomosyn, while the original is referred to as m-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain. This antibody is routinely tested by western blot analysis.

Molecular Weight: 130 kDa