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RAD9A antibody (RAD9 Homolog A (S. Pombe)) (AA 264-370)

Details for Product anti-RAD9A Antibody No. ABIN968473, Supplier: Log in to see
Antigen
  • MGC89062
  • RAD9
  • Rad9
  • rad9a
Epitope
AA 264-370
27
27
27
26
20
15
13
13
13
13
13
12
9
9
8
7
5
5
4
4
4
4
4
4
4
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
283
69
62
19
5
4
3
1
1
1
Host
Mouse
318
15
15
Clonality (Clone)
Monoclonal ()
Conjugate
This RAD9A antibody is un-conjugated
27
19
19
19
19
19
8
8
8
8
8
8
8
8
8
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
238
150
106
89
47
19
16
15
13
10
1
1
1
1
1
Supplier
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Immunogen Human hRAD9 aa. 264-370
Clone 56-hRad9
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name hRad9 (RAD9A Antibody Abstract)
Background Cell cycle checkpoints are regulatory mechanisms that prevent cell cycle progression in the presence of DNA damage or incompletely replicated DNA. Many of the genes required for cell-cycle arrest are also involved in DNA repair, apoptosis, and induction of transcription. In yeast and humans, hRAD9 plays a role in cell cycle arrest during the G2 phase before entry into mitosis. Phosphorylated hRAD9 is found in the nucleus after DNA damage, and forms DNA damage-responsive complexes with other putative checkpoint control proteins, such as hRAD1 and hHUS1. Expression of hRAD9 in S. pombe rad9::ura4+ cells restores resistance to the DNA synthesis inhibitor hydroxyurea and gamma rays. In addition, hRAD9 binds the anti-apoptotic proteins, Bcl-2 and Bcl-xL , and antisense hRAD9 RNA suppresses DNA-damage induced cell death. Thus, hRAD9 may be an important component of protein complexes that regulate cell cycle progression, as well as apoptosis, in response to DNA damage.
Molecular Weight 60 kDa
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-RAD9A antibody (RAD9 Homolog A (S. Pombe)) (AA 264-370) (ABIN968473) Western blot analysis of hRAD9 on a human endothelial cell lysate. Lane 1: 1:250, lan...
 image for anti-RAD9A antibody (RAD9 Homolog A (S. Pombe)) (AA 264-370) (ABIN968473) anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 264-370) antibody (Image 2)
Western Blotting (WB) image for anti-RAD9A antibody (RAD9 Homolog A (S. Pombe)) (AA 264-370) (ABIN968473) anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 264-370) antibody (Image 3)
Product cited in: Yoshida, Komatsu, Wang, Kufe: "c-Abl tyrosine kinase regulates the human Rad9 checkpoint protein in response to DNA damage." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3292-300, 2002

Komatsu, Miyashita, Hang, Hopkins, Zheng, Cuddeback, Yamada, Lieberman, Wang: "Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-xL and promotes apoptosis." in: Nature cell biology, Vol. 2, Issue 1, pp. 1-6, 2000

Volkmer, Karnitz: "Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 567-70, 1999

St Onge, Udell, Casselman, Davey: "The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1." in: Molecular biology of the cell, Vol. 10, Issue 6, pp. 1985-95, 1999

Lieberman, Hopkins, Nass, Demetrick, Davey: "A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 13890-5, 1997