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Topo IIalpha (AA 1245-1361) antibody

Details for Product No. ABIN968474, Supplier: Log in to see
Antigen
Epitope
AA 1245-1361
14
2
1
1
1
1
1
1
1
Reactivity
Human
23
3
2
1
Host
Mouse
22
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
BioImaging (BI), Immunofluorescence (IF), Western Blotting (WB)
13
11
9
6
3
2
1
Supplier
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Immunogen Human Topo IIalpha aa. 1245-1361
Clone 31-Topo IIa
Isotype IgG1
Characteristics 1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Topo II alpha
Background Eukaryotic DNA topoisomerase II, a ubiquitous ATP-dependent type II topoisomerase, is an essential nuclear enzyme in DNA replication and transcription, chromatin segregation, and cell cycle progression. These enzymes transiently break a pair of complementary strands in double-stranded DNA to form a gate for the passage of duplex DNA. Two isoforms of DNA topoisomerase II have been identified, topo IIalpha and topo IIß. These have a high degree of homology, except for some divergence in the C-terminal region. Both contain multiple bipartite nuclear localization sequences (NLS) that mediate their subnuclear localization. Topo IIalpha levels rise during late S phase and peak in G2-M, whereas topo IIß levels remain constant throughout the cell cycle. In addition, topo IIalpha is expressed in proliferating cells, while topo IIß is expressed in many tissues. The exact role of these two isoforms during cell proliferation is not known, however the differences in cellular expression implicate different physiological roles. Both isoforms may also be important targets for anticancer agents that exert cytotoxicity in proliferating cells via stabilization of a topo II-DNA complex. This antibody is routinely tested by Western blot analysis and immunofluorescent imaging.
Molecular Weight 170 kDa
Application Notes Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Topo IIalpha (AA 1245-1361) antibody (ABIN968474) Western blot analysis of Topo IIalpha on a HeLa lysate. Lane 1: 1:500, lane 2: 1:1000...
Immunofluorescence (IF) image for anti-Topo IIalpha (AA 1245-1361) antibody (ABIN968474) Immunofluorescent staining of HeLa cells. Cells were seeded in a 96 well imaging plat...
Product cited in: Mirski, Gerlach, Cole: "Sequence determinants of nuclear localization in the alpha and beta isoforms of human topoisomerase II." in: Experimental cell research, Vol. 251, Issue 2, pp. 329-39, 1999

Brown, Holden, Rahn, Perkins: "Immunohistochemical staining for DNA topoisomerase IIa in Hodgkin's disease." in: American journal of clinical pathology, Vol. 109, Issue 1, pp. 39-44, 1998

Herzog, Holmes, Tuschong, Ganapathi, Zwelling: "Absence of topoisomerase IIbeta in an amsacrine-resistant human leukemia cell line with mutant topoisomerase IIalpha." in: Cancer research, Vol. 58, Issue 23, pp. 5298-300, 1998

Background publications Tsai-Pflugfelder, Liu, Liu, Tewey, Whang-Peng, Knutsen, Huebner, Croce, Wang: "Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 19, pp. 7177-81, 1988