Adaptor-Related Protein Complex 3, delta 1 Subunit (AP3D1) (AA 627-731) antibody

Antigen: Adaptor-Related Protein Complex 3, delta 1 Subunit (AP3D1)

Synonyms: ADTD, hBLVR, mh, Ap3d, Bolvr, mocha, mBLVR1, AA407035, MGC140989, wu:fj51g01, si:ch211-129c21.6

Binding Site: AA 627-731

Clonality: Monoclonal (18)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968477

Additional Information

Alternative name: Adaptin delta

Immunogen: Human Adaptin delta

Format: Liquid

Isotype: IgG1

Clone: 18

Description: Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1 through AP-4), which are associated with the vesicle coats. These coatomers contain two large adaptin proteins (gamma, alpha, delta, epsilon and beta1, beta2, beta3, beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain (sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. In the AP-3 complex, adaptin delta contains an N-terminal head domain that has 25% identity with adaptins alpha and gamma, while its C-terminal hinge and ear domains are two-fold larger and more divergent as compared to the same domains in adaptins alpha and gamma. Adaptin delta is widely expressed and its mutation results in protein storage deficiencies within lysosomal-related organelles in platelets, melansomes, and synaptic vesicles. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 160 kDa

Comments:

Related Products: ABIN968535, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of Adaptin delta on a HeLa lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- Adaptin delta antibody.

Immunoflourescence staining of ES2 cells.

Publications

Product: Ooi, Moreira, DellAngelica et al.: "Altered expression of a novel adaptin leads to defective pigment granule biogenesis in the Drosophila eye color mutant garnet." in: The EMBO journal, Vol. 16, Issue 15, pp. 4508-18, 1997 (PubMed).

Le Borgne, Alconada, Bauer et al.: "The mammalian AP-3 adaptor-like complex mediates the intracellular transport of lysosomal membrane glycoproteins." in: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29451-61, 1998 (PubMed).

Zhen, Jiang, Feng et al.: "Abnormal expression and subcellular distribution of subunit proteins of the AP-3 adaptor complex lead to platelet storage pool deficiency in the pearl mouse." in: Blood, Vol. 94, Issue 1, pp. 146-55, 1999 (PubMed).

Blumstein, Faundez, Nakatsu et al.: "The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 20, pp. 8034-42, 2001 (PubMed).