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Caveolin antibody (pTyr14)

CAV Reactivity: Human, Mouse, Rat WB, IF, IHC (zinc) Host: Mouse Monoclonal 56-Caveolin unconjugated
Catalog No. ABIN968480
  • Target
    Caveolin (CAV)
    Binding Specificity
    pTyr14
    Reactivity
    Human, Mouse, Rat
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))
    Cross-Reactivity
    Mouse (Murine), Rat (Rattus)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Please refer to us for technical protocols.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Phosphorylated Human Caveolin-1 (Y14) Peptide
    Clone
    56-Caveolin
    Isotype
    IgG1
  • Comment

    Related Products: ABIN967389, ABIN968534

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Hill, Scherbakov, Schiefermeier, Baran, Hancock, Huber, Parton, Parat: "Reassessing the role of phosphocaveolin-1 in cell adhesion and migration." in: Traffic (Copenhagen, Denmark), Vol. 8, Issue 12, pp. 1695-705, (2007) (PubMed).

    Labrecque, Royal, Surprenant, Patterson, Gingras, Béliveau: "Regulation of vascular endothelial growth factor receptor-2 activity by caveolin-1 and plasma membrane cholesterol." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 334-47, (2003) (PubMed).

    Lee, Volonte, Galbiati, Iyengar, Lublin, Bregman, Wilson, Campos-Gonzalez, Bouzahzah, Pestell, Scherer, Lisanti: "Constitutive and growth factor-regulated phosphorylation of caveolin-1 occurs at the same site (Tyr-14) in vivo: identification of a c-Src/Cav-1/Grb7 signaling cassette." in: Molecular endocrinology (Baltimore, Md.), Vol. 14, Issue 11, pp. 1750-75, (2001) (PubMed).

    Ushio-Fukai, Hilenski, Santanam, Becker, Ma, Griendling, Alexander: "Cholesterol depletion inhibits epidermal growth factor receptor transactivation by angiotensin II in vascular smooth muscle cells: role of cholesterol-rich microdomains and focal adhesions in angiotensin II signaling." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 48269-75, (2001) (PubMed).

    Volonté, Galbiati, Pestell, Lisanti: "Cellular stress induces the tyrosine phosphorylation of caveolin-1 (Tyr(14)) via activation of p38 mitogen-activated protein kinase and c-Src kinase. Evidence for caveolae, the actin cytoskeleton, and focal adhesions as mechanical sensors of osmotic stres" in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8094-103, (2001) (PubMed).

    Glenney, Soppet: "Sequence and expression of caveolin, a protein component of caveolae plasma membrane domains phosphorylated on tyrosine in Rous sarcoma virus-transformed fibroblasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 21, pp. 10517-21, (1992) (PubMed).

  • Target
    Caveolin (CAV)
    Alternative Name
    Caveolin
    Background
    Caveolin (VIP21) localizes to non-clathrin membrane invaginations (caveolae) on the inner surface of the plasma membrane. In addition, it is present in the trans-Golgi network (TGN) and in apically and basolaterally destined transport vesicles. Caveolin is a transmembrane adaptor molecule that recognizes GPI-linked proteins and interacts with downstream cytoplasmic signaling molecules, such as src-family tyrosine kinases and hetero-trimeric G proteins. Caveolin forms large lipid-binding oligomers, which are thought to play a role in caveolae formation. It may also function as a scaffolding protein, which organizes signaling molecules. This functional role is supported by the fact that caveolin interacts directly with inactive ras and G-protein alpha subunits. Phosphorylation of caveolin at Tyr-14, Ser-88, and other residues in v-src transformed cells leads to flattening, aggregation, and fusion of caveolae and caveolae-derived vesicles. Thus, caveolin is the principle protein of caveolae and may be involved in v-src mediated cellular transformation.
    This antibody has also been reported to cross-react to paxillin in mouse embryonic fibroblasts (MEF), observable to migrate at ~ 68 kDa .
    Molecular Weight
    21-24 kDa
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