Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 110-230) antibody

Details for Product No. ABIN968487
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Synonyms cb34, Ap2m1, dpy-23, sb:cb34, zgc:55711, zgc:85653, wu:fa97a10, ap2m1, zgc:56643, AP2M1, AP50, CLAPM1, mu2, Ap50, Clapm1, ap2m1-a
AA 110-230
(19), (14), (12), (12), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(125), (48), (46), (3), (3), (1), (1), (1)
(96), (21), (12)
Clonality (Clone)
Monoclonal ()
(10), (10), (10), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Immunofluorescence (IF), Western Blotting (WB)
(98), (55), (34), (28), (20), (11), (9), (4), (2), (1), (1)
Pubmed 2 references available
Quantity 50 μg
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Immunogen Mouse AP50/mu2 aa. 110-230
Clone 31-AP50
Isotype IgG1
Cross-Reactivity Rat (Rattus), Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name AP50 (AP2M1 Antibody Abstract)
Background Sorting of integral membrane proteins is mediated vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large adaptin proteins (gamma, alpha, delta, epsilon, and beta1, beta2, beta3, beta4 respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, µ4 respectively) and one small chain ( sigma1, sigma2, sigma3, sigma4 respectively). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2, µ2 (AP50) interacts with integral membrane proteins via binding to tyrosine-based signals with the canonical motif YXXPhi. In addition, AP50/µ2 is required for both the assembly and the proton transport activity of vacuolar (H+)-ATPases in clathrin coated vesicles. Thus, AP50/µ2 may be involved in targeting integral membrane proteins that are sorted based on tyrosine-based signals and involved in assembly of functional ion channels associated with clathrin coated vesicles.
Molecular Weight 50 kDa

Related Products: ABIN968545, ABIN967389, ABIN968533

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 110-230) antibody (ABIN968487) Western blot analysis of AP50 on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500,...
Product cited in: Vecchi, Polo, Poupon et al.: "Nucleocytoplasmic shuttling of endocytic proteins." in: The Journal of cell biology, Vol. 153, Issue 7, pp. 1511-7, 2001 (PubMed).

Ohno, Stewart, Fournier et al.: "Interaction of tyrosine-based sorting signals with clathrin-associated proteins." in: Science (New York, N.Y.), Vol. 269, Issue 5232, pp. 1872-5, 1995 (PubMed).

Validation Images
Catalog No. ABIN968487
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