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Tumor Protein P53 Binding Protein 2 (TP53BP2) (AA 674-793) antibody

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Synonyms TP53BP2, AI449786, ASPP1, AW545810, Tp53bp2, Trp53bp2, p85, 53BP2, AI746547, ASPP2, PPP1R13A, X98550, wu:fa09h08, wu:fb04e08, zgc:85720, BBP, P53BP2
AA 674-793
(21), (16), (5), (4), (3), (3), (2), (2), (1), (1)
(62), (22), (22), (4), (2), (2), (1), (1), (1)
(56), (6)
Clonality (Clone)
Monoclonal ()
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Western Blotting (WB)
(45), (30), (11), (11), (8), (4), (3), (2)
Pubmed 3 references available
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Quantity 50 μg
Shipping to United States ( )
Immunogen Human 53BP2 aa. 674-793
Clone 19-53BP2
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name 53BP2 (TP53BP2 Antibody Abstract)
Background The p53 protein is critical to regulation of normal cell growth and is a suppressor of tumor cell proliferation. Inactivation of p53 by a number of mechanisms, such as missense mutations or interaction with oncogenic viral or cellular proteins, can result in tumor progression. In addition, Bcl2 and p53 are involved in apoptosis in an antagonistic fashion such that overexpressed Bcl2 inhibits p53-induced apoptosis. 53BP1 and 53BP2 were identified in a yeast two-hybrid screen of proteins that bind p53. Both 53BP1 and 53BP2 bind wild type p53, but not mutant p53 found in tumor cells. p53BP1 is localized to the cytoplasm and nucleus, while p53BP2 is found only in the cytoplasm. 53BP1 has BRCT motifs found in proteins involved in cell cycle control and DNA repair. DNA damage leads to 53BP1 hyperphosphorylation, which may be mediated by ATM. 53BP2 has four ankyrin repeats and a SH3 domain that are required for interactions with Bcl2 and p53. Overexpression of 53BP2 in 293 cells inhibits progression of the cell cycle in G2/M phase, while co-transfection of 53BP2 with p53 in H358 cells enhances p53-mediated transcriptional activation. The interaction between 53BP2 and p53 may be regulated by Bcl2, since competition experiments demonstrate that Bcl2 prevents p53 binding to 53BP2. In addition, 53BP2 can also bind the apoptotic-related p65 subunit of NFkappaB and this subunit can inhibit 53BP2-induced cell death.
Molecular Weight 137 kDa
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Tumor Protein P53 Binding Protein 2 (TP53BP2) (AA 674-793) antibody (ABIN968491) Western blot analysis of 53BP2 on human endothelial lysate. Lane 1: 1:250, lane 2: 1:...
 image for anti-Tumor Protein P53 Binding Protein 2 (TP53BP2) (AA 674-793) antibody (ABIN968491) Endothelial Cells
 image for anti-Tumor Protein P53 Binding Protein 2 (TP53BP2) (AA 674-793) antibody (ABIN968491) anti-Tumor Protein P53 Binding Protein 2 (TP53BP2) (AA 674-793) antibody (Image 3)
Western Blotting (WB) image for anti-Tumor Protein P53 Binding Protein 2 (TP53BP2) (AA 674-793) antibody (ABIN968491) anti-Tumor Protein P53 Binding Protein 2 (TP53BP2) (AA 674-793) antibody (Image 4)
Product cited in: Yang, Hori, Takahashi et al.: "NF-kappaB subunit p65 binds to 53BP2 and inhibits cell death induced by 53BP2." in: Oncogene, Vol. 18, Issue 37, pp. 5177-86, 1999 (PubMed).

Iwabuchi, Li, Massa et al.: "Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2." in: The Journal of biological chemistry, Vol. 273, Issue 40, pp. 26061-8, 1998 (PubMed).

Naumovski, Cleary: "The p53-binding protein 53BP2 also interacts with Bc12 and impedes cell cycle progression at G2/M." in: Molecular and cellular biology, Vol. 16, Issue 7, pp. 3884-92, 1996 (PubMed).

Catalog No. ABIN968491
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