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XRCC5 antibody (AA 103-315)

XRCC5 Reactivity: Human WB, IF Host: Mouse Monoclonal 7-Ku80 unconjugated
Catalog No. ABIN968493
  • Target See all XRCC5 Antibodies
    XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))
    Binding Specificity
    • 15
    • 14
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 103-315
    Reactivity
    • 95
    • 14
    • 11
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 78
    • 14
    • 2
    • 1
    Mouse
    Clonality
    • 70
    • 23
    Monoclonal
    Conjugate
    • 65
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This XRCC5 antibody is un-conjugated
    Application
    • 81
    • 41
    • 39
    • 30
    • 20
    • 12
    • 12
    • 10
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Ku80 aa. 103-315
    Clone
    7-Ku80
    Isotype
    IgG1
    Top Product
    Discover our top product XRCC5 Primary Antibody
  • Comment

    Related Products: ABIN968533, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Kim, Kim, Han, Jeong, Chung, Kang, Li: "Ku autoantigen affects the susceptibility to anticancer drugs." in: Cancer research, Vol. 59, Issue 16, pp. 4012-7, (1999) (PubMed).

    Majumder, Ghoshal, Li, Jacob: "Hypermethylation of metallothionein-I promoter and suppression of its induction in cell lines overexpressing the large subunit of Ku protein." in: The Journal of biological chemistry, Vol. 274, Issue 40, pp. 28584-9, (1999) (PubMed).

    Smith, Jackson: "The DNA-dependent protein kinase." in: Genes & development, Vol. 13, Issue 8, pp. 916-34, (1999) (PubMed).

  • Target
    XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))
    Alternative Name
    Ku80 (XRCC5 Products)
    Synonyms
    AI314015 antibody, Ku80 antibody, Ku86 antibody, Kup80 antibody, KARP-1 antibody, KARP1 antibody, KU80 antibody, KUB2 antibody, NFIV antibody, xrcc5-a antibody, XRCC5 antibody, X-ray repair cross complementing 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 L homeolog antibody, XRCC5 antibody, Xrcc5 antibody, xrcc5.L antibody
    Background
    DNA-dependent protein kinase (DNA-PK) is a trimeric enzyme that contains a catalytic subunit of 350 kDa (DNA-PKcs) and a heterodimeric regulatory subunit of 70 kDa (Ku70) and 86 kDa (Ku80). DNA-PKcs is inactive alone and depends on the regulatory subunit for subcellular localization and kinase activity. The DNA-PKcs/Ku70/Ku80 complex is involved in V(D)J recombination and DNA double-stranded break repair. Ku70 and Ku80 are abundant nuclear DNA-binding proteins. Besides functioning in a complex with DNA-PKcs, Ku proteins may act in multiple cellular processes including transcriptional regulation, ATPase and helicase activity, alteration in chromatin structure, cell cycle regulation, and maintenance of telomere length. In rat fibroblasts, Ku80 overexpression leads to hypermethylation and silencing of metallothionein gene expression. Ku70-/- and Ku80-/- cells are less resistant to anticancer drug-induced apoptosis, suggesting a role for Ku proteins in the prevention of apoptotic signaling. Thus, Ku70 and Ku80 may have multiple roles during DNA transcription, repair, and maintenance depending on the protein interactions that are involved.
    Molecular Weight
    80 kDa
    Pathways
    DNA Damage Repair
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