Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 252-445) antibody

Antigen: Apoptotic Peptidase Activating Factor 1 (APAF1)

Synonyms: CED4, APAF-1, DKFZp781B1145, fog, Apaf-1, Apaf1l, KIAA0413, mKIAA0413, 6230400I06Rik, ARK, Apaf1, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, Hac-1, Hac-1/Dark, Hac1, T1, anon-53Fa, arc, dApaf-1/DARK/HAC-1, dApaf1, dapaf, dapaf-1L, dapaf-1S, dark/dapaf-1/hac-1, DmelCG6829, CG6829, apaf-1, MGC80868, APAF1

Binding Site: AA 252-445

Clonality: Monoclonal (24)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968497

Additional Information

Alternative name: Apaf-1

Immunogen: Human Apaf-1

Format: Liquid

Isotype: IgG1

Clone: 24

Description: The process of apoptosis requires the activation of aspartate-specific cystenine proteases in the caspase family. Group I caspases (1,4,5) cleave at (W/L)EHD tetrapeptide motifs, while group II caspases (2,3,7) cleave the DEXD tetrapeptide motif. Group III caspases (6,8,9) are activators of other caspases via cleavage of (I/V)EXD tetrapeptide sequences. Apoptotic protease-activating factor-1 (Apaf-1), cytochrome c, and dATP activate caspase-9, which in turn, initiates the post-mitochondrial-mediated caspase cascade that includes caspase-2, 3, 6, 7, 8 and 10. Apaf-1 is a soluble protein with a short N-terminal caspase recruitment domain (CARD), a central CED-4 homology domain, and 12 WD-40 repeats that may be involved in protein-protein interactions. During apoptosis, a large (700 kDa) aposome complex containing Apaf-1, cytochrome c, caspase-3, 7, and 9, and a smaller (200-300 kDa) microaposome complex containing caspase-3 and 7 exhibit higher cleavage activity than free caspase heterotetramers. Thus Apaf-1 is a component of the large aposome complex, which functions in caspase activation leading to caspase-dependent proteolytic events and apoptosis.
Synonyms: Apoptotic Protease Activating Factor-1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 130 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Synonyms: CED4, APAF-1, DKFZp781B1145, fog, Apaf-1, Apaf1l, KIAA0413, mKIAA0413, 6230400I06Rik, ARK, Apaf1, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, Hac-1, Hac-1/Dark, Hac1, T1, anon-53Fa, arc, dApaf-1/DARK/HAC-1, dApaf1, dapaf, dapaf-1L, dapaf-1S, dark/dapaf-1/hac-1, DmelCG6829, CG6829, apaf-1, MGC80868, APAF1

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of Apaf-1 on a human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- Apaf-1 antibody.

Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75).

anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 252-445) antibody (Image 3)

Publications

Product: Slee, Harte, Kluck et al.: "Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner." in: The Journal of cell biology, Vol. 144, Issue 2, pp. 281-92, 1999 (PubMed).

Soengas, Alarcón, Yoshida et al.: "Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 156-9, 1999 (PubMed).

Hu, Benedict, Ding et al.: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999 (PubMed).

Cain, Brown, Langlais et al.: "Caspase activation involves the formation of the aposome, a large (approximately 700 kDa) caspase-activating complex." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22686-92, 1999 (PubMed).

Li, Trudel, Wogan: "Nitric oxide-induced genotoxicity, mitochondrial damage, and apoptosis in human lymphoblastoid cells expressing wild-type and mutant p53." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 16, pp. 10364-9, 2002 (PubMed).