Fused in Sarcoma (FUS) (AA 2-117) antibody

Antigen: Fused in Sarcoma (FUS)

Binding Site: AA 2-117

Clonality: Monoclonal (15)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968509

Additional Information

Alternative name: TLS

Immunogen: Human TLS .

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 15

Description: Chromosomal translocations in leukemias and malignancies of non-hematopoietic tissues usually result in the fusion of genes and the production of novel fusion proteins. Translocated in liposarcoma (TLS/FUS) was identified through its fusion to the transcription factor CHOP in human myxoid liposarcoma. In addition, the fusion of TLS to the transcription factor ERG is involved in human acute myeloid leukemia. TLS contains an N-terminal Gln-, Ser-, and Tyr-rich region (QSY) that is thought to be a potent transactivator when fused with transcription factors. In its C-terminus, TLS contains a ribonucleoprotein consensus sequence (RNP-CS) and Arg-Gly-Gly (RGG) repeats that have been implicated in RNA binding. This feature allows it to function in shuttling of RNA from the nucleus. TLS also interacts with Ser-Arg proteins that regulate RNA splicing. Interestingly, TLS also interacts with the DNA binding domains of the estrogen, thyroid hormone, and glucocorticoid receptors. Thus, TLS is a multifunctional protein that is involved in RNA transport, nuclear receptor function, and gene transactivation when fused with transcription factors. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 65 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of TLS on a Jurkat cell lysate. Lane 1: 1:250, Lane 2: 1:500, Lane 3: 1:1000 dilution of the anti- TLS antibody.

Publications

Product: Zinszner, Sok, Immanuel et al.: "TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling." in: Journal of cell science, Vol. 110 ( Pt 15), pp. 1741-50, 1997 (PubMed).

Powers, Mathur, Raaka et al.: "TLS (translocated-in-liposarcoma) is a high-affinity interactor for steroid, thyroid hormone, and retinoid receptors." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 1, pp. 4-18, 1998 (PubMed).

Hallier, Lerga, Barnache et al.: "The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS." in: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 4838-42, 1998 (PubMed).

Yang, Embree, Tsai et al.: "Oncoprotein TLS interacts with serine-arginine proteins involved in RNA splicing." in: The Journal of biological chemistry, Vol. 273, Issue 43, pp. 27761-4, 1998 (PubMed).

Morohoshi, Ootsuka, Arai et al.: "Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes." in: Gene, Vol. 221, Issue 2, pp. 191-8, 1998 (PubMed).