p95PKL (AA 140-472) antibody

Details for Product No. ABIN968512, Supplier: Log in to see
AA 140-472
Human, Mouse (Murine), Rat (Rattus)
Clonality (Clone)
Monoclonal ()
Immunofluorescence (IF), Western Blotting (WB)
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Immunogen Chicken p95PKL aa. 140-472
Clone 13-P95PKL
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine), Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background Paxillin is involved in the integration of growth factor- and adhesion-mediated signal transduction pathways at focal adhesion sites. The LD motif of paxillin has been implicated in its binding to FAK, vinculin, and paxillin-kinase linker (p95PKL). p95PKL couples paxillin to a protein complex containing p21 GTPase-activated kinase (PAK), Nck, and the guanine nucleotide exchange factor, PIX. The sequence of p95PKL, highly homologous to human GIT1 and GIT2, predicts a structure that contains an N-terminal ARF-GAP domain containing a Zn2+ finger, three ankyrin repeats, an EF hand domain, and two IQ motifs. p95PKL binds to both paxillin and PIX and colocalizes with paxillin at focal adhesion sites. Overexpression of a paxillin LD4 deletion mutant in neuroblastoma cells inhibits insulin-like growth factor-1 mediated lamellipodia formation. Microinjection of this mutant into NIH3T3 cells has been reported to decrease migration into a wound. These alterations in cell motility are thought to involve PAK-mediated regulation of the actin cytoskeleton, since the LD4 motif of paxillin is required for p95PKL-dependent recruitment of PAK/PIX complexes to paxillin-containing focal adhesions sites. This antibody has been reported to recognize GIT1 and GIT2 proteins. This antibody is routinely tested by western blot analysis.
Synonyms: Paxillin-Kinase Linker
Molecular Weight 95 kDa

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-p95PKL (AA 140-472) antibody (ABIN968512) Western blot analysis of p95PKL/GIT on a rat cerebrum lysate. Lane 1: 1:500, lane 2: ...
Immunofluorescence (IF) image for anti-p95PKL (AA 140-472) antibody (ABIN968512) Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells, ATCC CRL...
Western Blotting (WB) image for anti-p95PKL (AA 140-472) antibody (ABIN968512) anti-p95PKL (AA 140-472) antibody (Image 3)
Product cited in: Ku, Yablonski, Manser, Lim, Weiss: "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT." in: The EMBO journal, Vol. 20, Issue 3, pp. 457-65, 2001 (PubMed).

Turner, Brown, Perrotta, Riedy, Nikolopoulos, McDonald, Bagrodia, Thomas, Leventhal: "Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling." in: The Journal of cell biology, Vol. 145, Issue 4, pp. 851-63, 1999 (PubMed).