p95PKL (AA 140-472) antibody

Details for Product No. ABIN968512
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Antigen
Epitope
AA 140-472
Reactivity
Chicken
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF)
Pubmed 2 references available
Quantity 50 μg
Options
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Catalog No. ABIN968512
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Immunogen Chicken p95PKL
Clone 7H3
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine), Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background Paxillin is involved in the integration of growth factor- and adhesion-mediated signal transduction pathways at focal adhesion sites. The LD motif of paxillin has been implicated in its binding to FAK, vinculin, and paxillin-kinase linker (p95PKL). p95PKL couples paxillin to a protein complex containing p21 GTPase-activated kinase (PAK), Nck, and the guanine nucleotide exchange factor, PIX. The sequence of p95PKL, highly homologous to human GIT1 and GIT2, predicts a structure that contains an N-terminal ARF-GAP domain containing a Zn2+ finger, three ankyrin repeats, an EF hand domain, and two IQ motifs. p95PKL binds to both paxillin and PIX and colocalizes with paxillin at focal adhesion sites. Overexpression of a paxillin LD4 deletion mutant in neuroblastoma cells inhibits insulin-like growth factor-1 mediated lamellipodia formation. Microinjection of this mutant into NIH3T3 cells has been reported to decrease migration into a wound. These alterations in cell motility are thought to involve PAK-mediated regulation of the actin cytoskeleton, since the LD4 motif of paxillin is required for p95PKL-dependent recruitment of PAK/PIX complexes to paxillin-containing focal adhesions sites. This antibody has been reported to recognize GIT1 and GIT2 proteins. This antibody is routinely tested by western blot analysis.
Synonyms: Paxillin-Kinase Linker
Molecular Weight 95 kDa
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-p95PKL (AA 140-472) antibody Western blot analysis of p95PKL/GIT on a rat cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti- p95PKL/GIT antibody.
anti-p95PKL (AA 140-472) antibody (2) Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells, ATCC CRL-1658).
Product cited in: Turner, Brown, Perrotta et al.: "Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling." in: The Journal of cell biology, Vol. 145, Issue 4, pp. 851-63, 1999 (PubMed).

Ku, Yablonski, Manser et al.: "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT." in: The EMBO journal, Vol. 20, Issue 3, pp. 457-65, 2001 (PubMed).

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