Ikappa B epsilon (AA 200-211) antibody

Antigen: Ikappa B epsilon

Binding Site: AA 200-211

Clonality: Monoclonal (21)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968522

Additional Information

Immunogen: Human IkappaBepsilon

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG2a

Clone: 21

Description: NF-kappaB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It consists of homo- and heterodimeric proteins that belong to the Rel family of transcription factors. Rel proteins such as p50, p52, p65 (RelA), c-Rel, and RelB contain a Rel homology region (RHR) that includes DNA-binding and dimerization domains and a nuclear localization signal. Inactive NF-kappaB complexes are associated with their inhibitory IkappaB proteins. Several IkappaB proteins have been characterized including IkappaBalpha, IkappaBbeta, and IkappaBepsilon. They contain ankyrin repeats that interact with Rel RHR regions to form complexes in the cytoplasm that prevent nuclear translocation of NF-kappaB. In response to stimuli such as cytokines, LPS, and viral infections, IkappaB proteins are phosphorylated at critical residues by IkappaB kinases (IKKalpha and IKKbeta) leading to proteolysis of IkappaB proteins. This frees the heterodimeric NF-kappaB to form a heterotetramer that translocates to the nucleus and acts as a transcriptional activator. IkappaBepsilon may function primarily in the cytoplasm where it sequesters p65 and c-Rel, while IkappaBalpha and IkappaBbeta have additional functions that involve entering the nucleus to inhibit Rel DNA binding.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 45 kDa

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of IkappaBepsilon on A431 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of IkappaBepsilon.

HeLa

anti-Ikappa B epsilon (AA 200-211) antibody (Image 3)

Publications

Product: Whiteside, Epinat, Rice et al.: "I kappa B epsilon, a novel member of the I kappa B family, controls RelA and cRel NF-kappa B activity." in: The EMBO journal, Vol. 16, Issue 6, pp. 1413-26, 1997 (PubMed).

Simeonidis, Liang, Chen et al.: "Cloning and functional characterization of mouse IkappaBepsilon." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 26, pp. 14372-7, 1998 (PubMed).

Page, Fischer, Baumgartner et al.: "4-Hydroxynonenal prevents NF-kappaB activation and tumor necrosis factor expression by inhibiting IkappaB phosphorylation and subsequent proteolysis." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 11611-8, 1999 (PubMed).

Fischer, Page, Weber et al.: "Differential effects of lipopolysaccharide and tumor necrosis factor on monocytic IkappaB kinase signalsome activation and IkappaB proteolysis." in: The Journal of biological chemistry, Vol. 274, Issue 35, pp. 24625-32, 1999 (PubMed).