NEDD4 antibody (AA 173-262)

Catalog No. ABIN968557

Product Details anti-NEDD4 Antibody

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Binding Specificity: AA 173-262

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEDD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Nedd4 aa. 173-262

Clone: 15-Nedd4

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-NEDD4 antibody (ABIN968557)

Peruzzi, Prisco, Morrione, Valentinis, Baserga: "Anti-apoptotic signaling of the insulin-like growth factor-I receptor through mitochondrial translocation of c-Raf and Nedd4." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 25990-6, (2001) (PubMed).

Harvey, Dinudom, Komwatana, Jolliffe, Day, Parasivam, Cook, Kumar: "All three WW domains of murine Nedd4 are involved in the regulation of epithelial sodium channels by intracellular Na+." in: The Journal of biological chemistry, Vol. 274, Issue 18, pp. 12525-30, (1999) (PubMed).

Plant, Yeger, Staub, Howard, Rotin: "The C2 domain of the ubiquitin protein ligase Nedd4 mediates Ca2+-dependent plasma membrane localization." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32329-36, (1998) (PubMed).

Hatakeyama, Jensen, Weissman: "Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases." in: The Journal of biological chemistry, Vol. 272, Issue 24, pp. 15085-92, (1997) (PubMed).

Target Details for NEDD4

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Alternative Name: Nedd4 (NEDD4 Products)

Synonyms: NEDD4-1 antibody, RPF1 antibody, AA959633 antibody, AL023035 antibody, AU019897 antibody, E430025J12Rik antibody, Nedd4-1 antibody, Nedd4a antibody, mKIAA0093 antibody, CG32184 antibody, CG42279 antibody, CG7555 antibody, DNedd4 antibody, Dmel\\CG42279 antibody, Dmel_CG32184 antibody, Dmel_CG7555 antibody, dNedd4 antibody, dNedd4-1 antibody, nedd4 antibody, GB16853 antibody, NEDD4 antibody, Nedd4 antibody, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase antibody, neural precursor cell expressed, developmentally down-regulated 4 antibody, CG42279 gene product from transcript CG42279-RJ antibody, E3 ubiquitin-protein ligase Nedd-4 antibody, E3 ubiquitin-protein ligase NEDD4 antibody, NEDD4 antibody, Nedd4 antibody, LOC411723 antibody, LOC100457150 antibody, nedd4 antibody, LOC100547485 antibody

Background: Regulated protein degradation involves conjugation of ubiquitin to proteins for degradation by the 26S proteasome. Protein ubiquitination is the end product of a multienzyme cascade that involves sequential thiol-ester bond interactions between ubiquitin and enzymes termed E1-E3. Neuronal precursor cell-expressed developmentally down-regulated 4 (Nedd4) is a multimodular ubiquitin protein ligase E3 that contains a Ca 2+-lipid binding domain (CaLB), 3 WW domains, and a C-terminal ubiquitin protein ligase hect domain. The CaLB is characteristic of C2 domain-containing proteins, such as PKC, phospholipase C, phospholipase A2, and synaptogamin. Nedd4 is expressed differentially in the fetal and adult brain and its highest expression is in lung and kidney. Although it localizes in the cytoplasm, increased intracellular Ca 2+ induces plasma membrane association of Nedd4. In addition, increases in intracellular Na+ leads to Nedd4 binding to epithelial Na+ channels via its WW domains, which results in targeting of Na+ channels for degradation. Thus, the subcellular localization and protein interactions of Nedd4 are regulated in a Ca2+- and Na+-dependent manner. This antibody is routinely tested by western blot analysis.

Molecular Weight: 110 kDa

Pathways: Notch Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2