phosphodiesterase 5A, CGMP-Specific (PDE5A) (AA 2-108) antibody

Antigen: phosphodiesterase 5A, CGMP-Specific (PDE5A)

Synonyms: CN5A, PDE5, PDE5A1, CGB-PDE, PDE5A2, Cn5n, Pde5, Cgbpde, Pde5a1, PDE5A, cn5a, pde5, pde5a1, cgb-pde, MGC146818

Binding Site: AA 2-108

Clonality: Monoclonal (54)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968564

Additional Information

Alternative name: cGB-PDE

Immunogen: Rat cGB-PDE

Format: Liquid

Isotype: IgG1

Clone: 54

Description: CGMP is involved in nitric oxide signaling, as well as cell signaling associated with natriuretic peptides and guanylins. Intracellular targets for cGMP include cGMP-dependent protein kinase, cyclic nucleotide-gate ion channels, and cGMP-Binding PhosphoDiesterases (cGB-PDE). Tissue levels of cGMP are determined by guanylyl cyclases that catalyze the formation of cGMP and cyclic nucleotide PDEs that catalyze the breakdown of cGMP. In the PDE superfamily PDE2, PDE5, PDE6, and PDE10 have dimeric structures that contain highly cGMP-specific allosteric cGMP binding sites in addition to a phosphodiesterase catalytic site. PDE5 is highly expressed in aorta and lung, but is also expressed in intestine, kidney, adrenal gland, cerebellum, and cerebrum. In the cerebellum, PDE5 is expressed in the Purkinje cell layer during neonatal development. PDE5 is also abundant in vascular smooth muscle cells where it is involved in regulating cGMP levels and vascular smooth muscle tonicity. Thus, PDE5 is important for regulating cGMP levels in both neuronal and non-neuronal tissues that utilize cGMP signaling pathways for the regulation of cell function.
Synonyms: PDE5, cGMP-Binding PhosphoDiesterases

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 85-95 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of cGB-PDE on a rat lung lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-rat cGB-PDE antibody.

Publications

Product: Kotera, Yanaka, Fujishige et al.: "Expression of rat cGMP-binding cGMP-specific phosphodiesterase mRNA in Purkinje cell layers during postnatal neuronal development." in: European journal of biochemistry / FEBS, Vol. 249, Issue 2, pp. 434-42, 1997 (PubMed).

Kotera, Fujishige, Akatsuka et al.: "Novel alternative splice variants of cGMP-binding cGMP-specific phosphodiesterase." in: The Journal of biological chemistry, Vol. 273, Issue 41, pp. 26982-90, 1998 (PubMed).

Corbin, Francis: "Cyclic GMP phosphodiesterase-5: target of sildenafil." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 13729-32, 1999 (PubMed).