KIF3A antibody (AA 563-671)

Catalog No. ABIN968567

Product Details anti-KIF3A Antibody

Target: KIF3A (Kinesin Family Member 3A (KIF3A))

Binding Specificity: AA 563-671

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KIF3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Characteristics: 1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human KIF3A aa. 563-671

Clone: 28-KIF3A

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-KIF3A antibody (ABIN968567)

Marszalek, Ruiz-Lozano, Roberts, Chien, Goldstein: "Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 9, pp. 5043-8, (1999) (PubMed).

Muresan, Lyass, Schnapp: "The kinesin motor KIF3A is a component of the presynaptic ribbon in vertebrate photoreceptors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 3, pp. 1027-37, (1999) (PubMed).

Hirokawa: "Kinesin and dynein superfamily proteins and the mechanism of organelle transport." in: Science (New York, N.Y.), Vol. 279, Issue 5350, pp. 519-26, (1998) (PubMed).

Target Details for KIF3A

Target: KIF3A (Kinesin Family Member 3A (KIF3A))

Alternative Name: KIF3A (KIF3A Products)

Synonyms: FLA10 antibody, KLP-20 antibody, 111-11-71 antibody, 111-11-86 antibody, AW124694 antibody, Kif3 antibody, Kifl antibody, Kns3 antibody, kinesin family member 3A antibody, kinesin family member 3a antibody, KIF3A antibody, Kif3a antibody

Background: The ability of the kinesin superfamily of motor proteins to hydrolyze ATP as they move progressively along microtubules is important for organelle transport and cell division. Kinesins are grouped according to the location of the motor domain in the N-terminal, middle, or C-terminal region of the protein. A family of N-terminal motor domain kinesin proteins includes KIF3A/3B, KRP85/95, and Klp68d/64. These proteins form heterodimeric two-headed motors that interact with kinesin associated protein (KAP). The KIF3A/3B/KAP3 heterotrimeric motor has plus-end directed microtubule sliding activity. This heterotrimeric motor is expressed ubiquitously and is used for anterograde transport of membranous organelles. KIF3A- and KIF3B-deficient mice have defects in the formation of the cilia located in the embryonic node. In addition, KIF3A localizes to the basal body of the connecting cilium axoneme and to vesicles at the pre-synaptic ribbon in photoreceptor cells. Thus, KIF3 motors are involved in microtubule-based formation and movement of cilia and the transport of synaptic vesicles. This antibody is routinely tested by the Western blot analysis.

Molecular Weight: 80/85 kDa

Pathways: Hedgehog Signaling