Coagulation Factor II (thrombin) Receptor (F2R) (AA 33-153) antibody

Antigen: Coagulation Factor II (thrombin) Receptor (F2R)

Synonyms: TR, HTR, CF2R, PAR1, PAR-1, Par1, TRGPC, MGC93622

Binding Site: AA 33-153

Clonality: Monoclonal (14)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968574

Additional Information

Alternative name: Thrombin Receptor

Immunogen: Human Thrombin Receptor/PAR1

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 14

Description: Thrombin is a serine protease that regulates the activity of many cell types including the activation of platelets after vascular injury. Thrombin functions are mediated by G-protein-coupled protease-activated receptors that are homologous to substance P and thyrotropin receptors. Thrombin receptors (PAR1, PAR3, and PAR4) are seven transmembrane domain proteins with large N-terminal exodomains that contain a thrombin cleavage site (LDPR/S). Thrombin binds to the exodomain and cleaves the peptide bond between Arg-41 and Ser-42. This unmasks a new N-terminus with the sequence SFLLRN that acts as a tethered ligand. SFLLRN binds to the body of the thrombin receptor leading to irreversable activation. Both phosphorylation of the thrombin receptor and internalization may uncouple downstream signaling. PAR1 protein is expressed in vascular endothelial cells, smooth muscle cells, and macrophages, while PAR1 mRNA is expressed at higher levels in neonatal rat brain and lower levels in skeletal muscle, liver, and kidney. Thus, G-protein signaling via thrombin receptors, like PAR1, may be important for a diverse array of cellular functions, such as platelet activation and neural development. This antibody is routinely tested by western blot analysis.
Synonyms: Protease Activated Receptor-1 (PAR1)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 47 kDa

Comments:

Related Products: ABIN968586, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Chromatin and Nuclear Signaling, Cell Cycle, Angiogenesis, Coagulation

Restrictions: For Research Use only

Images

Western blot analysis of Thrombin Receptor/PAR1 on a K-562 cell lysate (Human bone marrow myelogenous leukemia, ATCC CCL-243). Lane 1: 1:250, lane 2: 1, 500, lane 3: 1: 1000 dilution of the mouse anti- Thrombin Receptor/PAR1 antibody.

Publications

Product: Nelken, Soifer, OKeefe et al.: "Thrombin receptor expression in normal and atherosclerotic human arteries." in: The Journal of clinical investigation, Vol. 90, Issue 4, pp. 1614-21, 1992 (PubMed).

Vu, Hung, Wheaton et al.: "Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation." in: Cell, Vol. 64, Issue 6, pp. 1057-68, 1991 (PubMed).

Niclou, Suidan, Brown-Luedi et al.: "Expression of the thrombin receptor mRNA in rat brain." in: Cellular and molecular biology (Noisy-le-Grand, France), Vol. 40, Issue 3, pp. 421-8, 1994 (PubMed).

Coughlin: "How the protease thrombin talks to cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11023-7, 1999 (PubMed).