Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

KSR1 antibody (AA 90-203)

KSR1 Reactivity: Mouse, Rat WB, IF Host: Mouse Monoclonal 15-KSR unconjugated
Catalog No. ABIN968599
  • Target See all KSR1 Antibodies
    KSR1 (Kinase Suppressor of Ras 1 (KSR1))
    Binding Specificity
    • 16
    • 15
    • 15
    • 8
    • 8
    • 8
    • 7
    • 7
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 90-203
    Reactivity
    • 86
    • 49
    • 21
    • 1
    Mouse, Rat
    Host
    • 98
    • 2
    Mouse
    Clonality
    • 93
    • 7
    Monoclonal
    Conjugate
    • 36
    • 10
    • 9
    • 8
    • 7
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This KSR1 antibody is un-conjugated
    Application
    • 61
    • 51
    • 22
    • 13
    • 13
    • 9
    • 7
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Rat (Rattus)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Mouse KSR-1 aa. 90-203
    Clone
    15-KSR
    Isotype
    IgG1
    Top Product
    Discover our top product KSR1 Primary Antibody
  • Comment

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Stewart, Sundaram, Zhang, Lee, Han, Guan: "Kinase suppressor of Ras forms a multiprotein signaling complex and modulates MEK localization." in: Molecular and cellular biology, Vol. 19, Issue 8, pp. 5523-34, (1999) (PubMed).

    Michaud, Therrien, Cacace, Edsall, Spiegel, Rubin, Morrison: "KSR stimulates Raf-1 activity in a kinase-independent manner." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 12792-6, (1998) (PubMed).

    Zhang, Yao, Delikat, Bayoumy, Lin, Basu, McGinley, Chan-Hui, Lichenstein, Kolesnick: "Kinase suppressor of Ras is ceramide-activated protein kinase." in: Cell, Vol. 89, Issue 1, pp. 63-72, (1997) (PubMed).

    Therrien, Chang, Solomon, Karim, Wassarman, Rubin: "KSR, a novel protein kinase required for RAS signal transduction." in: Cell, Vol. 83, Issue 6, pp. 879-88, (1996) (PubMed).

  • Target
    KSR1 (Kinase Suppressor of Ras 1 (KSR1))
    Alternative Name
    KSR-1 (KSR1 Products)
    Synonyms
    CG2899 antibody, Dmel\\CG2899 antibody, EK3-1 antibody, KSR antibody, KSR-1 antibody, Ksr antibody, Ksr-1 antibody, SR3-1 antibody, dKsr antibody, l(3)j5E2 antibody, AW492498 antibody, B-KSR1 antibody, D11Bhm183e antibody, D11Bhm184e antibody, RSU2 antibody, hb antibody, kinase suppressor of ras 1 antibody, kinase suppressor of ras antibody, KSR1 antibody, ksr antibody, Ksr1 antibody
    Background
    Proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. In mammalian cells there are four true Ras proteins (encoded by Ha-ras, N-ras, Ki-rasA, and Ki-rasB). These proteins relay signals from tyrosine kinases at the plasma membrane to the nucleus via a network of Ser/Thr kinases that includes the MAP kinase (Raf-MEK-ERK) pathway. Kinase suppressor of Ras (KSR-1) was discovered in a genetic screen to identify mutations that suppress constitutively active Ras mutants. KSR-1 contains five domains that are conserved areas (CA) in Drosophila, mouse, and human. CA1 is a unique domain, CA2 is a proline-rich domain, CA3 is a cysteine-rich domain, CA4 is a Ser/Thr rich region, and CA5 contains a C-terminal kinase domain similar to that of Raf. In COS7 cells, ceramide induces the autophosphorylation of KSR-1 and KSR-1-induced phosphorylation and activation of Raf. In addition, overexpression of KSR-1 in 293T cells leads to recruitment of MEK to a 700 kDa protein complex and translocates MEK from the soluble to the membrane-associated fraction. Thus, KSR-1 may have important kinase and scaffolding activities during Ras-related signaling. This antibody is routinely tested by western blot analysis.
    Molecular Weight
    115 kDa
You are here:
Support