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Superoxide Dismutase 2, Mitochondrial (SOD2) (AA 114-220) antibody

Details for Product No. ABIN968602, Supplier: Log in to see
Antigen
  • cb463
  • CG8905
  • Dmel\\CG8905
  • dSOD2
  • GB14346
  • IPOB
  • LOC100101896
  • MGC88869
  • Mito SOD
  • mitSOD2
  • Mn-SOD
  • Mn-SOD2
  • mnSOD
  • Mn SOD
  • MNSOD
  • MnSOD
  • MnSODII
  • Mn[2+]SOD
  • MVCD6
  • SOD
  • SOD-2
  • Sod-2
  • sod2
  • Sod2
  • SOD2
  • wu:fj33b01
  • zgc:73051
Epitope
AA 114-220
20
13
8
7
7
7
5
4
2
2
2
2
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
229
128
107
51
50
50
49
47
46
46
46
46
45
25
22
20
18
18
18
4
1
1
1
1
1
1
Host
Mouse
181
64
20
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
13
12
6
6
6
5
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
226
150
128
109
45
42
32
11
10
7
5
4
Supplier
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Immunogen Mouse MnSOD aa. 114-220
Clone 19-MnSOD
Isotype IgG1
Cross-Reactivity Human, Dog (Canine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MnSOD (SOD2 Antibody Abstract)
Background Superoxide dismutase (SOD), catalase, and glutathione peroxidase are necessary for preventing the intracellular accumulation of reactive oxygen species. Three forms of SOD include the cytosolic Cu/Zn2+ SOD, the extracelluar Cu/Zn[2+] SOD, and the mitochondrial manganese (Mn) superoxide dismutase (MnSOD). In the mitochondria, MnSOD catalyzes the dismutation of two superoxide radicals, producing H2O2 and oxygen. Following protein synthesis, MnSOD is post-transcriptionally modified for transport from the cytosol into the mitochondria. In rat hepatocytes, H2O2 exposure induces MnSOD expression, while in rat lung, exposure to smoke induces expression in bronchial epithelial cells. In addition, MnSOD expression is required for PC12 cell resistance to nitric oxide (NO) toxicity and for nNOS neuron resistance to NMDA-induced NO toxicity. Superoxide radical anions may induce transcriptional activation of the MnSOD gene through PKC phosphorylation and activation of a CREB-1/ATF-1-like factor. Thus, MnSOD removal of reactive oxygen species created by exposure to toxic stimuli is thought to be critical for the survival of a variety of cell types. This antibody is routinely tested by western blot analysis.
Synonyms: Manganese Superoxide Dismutase
Molecular Weight 25 kDa
Comment

Related Products: ABIN968542, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Superoxide Dismutase 2, Mitochondrial (SOD2) (AA 114-220) antibody (ABIN968602) Western blot analysis of MnSOD on a mouse kidney lysate. Lane 1: 1:1000, lane 2: 1:20...
Product cited in: Kim, Roe, Chock, Yim: "Transcriptional activation of the human manganese superoxide dismutase gene mediated by tetradecanoylphorbol acetate." in: The Journal of biological chemistry, Vol. 274, Issue 52, pp. 37455-60, 2000 (PubMed).

Ambrosone, Freudenheim, Thompson, Bowman, Vena, Marshall, Graham, Laughlin, Nemoto, Shields: "Manganese superoxide dismutase (MnSOD) genetic polymorphisms, dietary antioxidants, and risk of breast cancer." in: Cancer research, Vol. 59, Issue 3, pp. 602-6, 1999 (PubMed).

Gilks, Price, Wright, Churg: "Antioxidant gene expression in rat lung after exposure to cigarette smoke." in: The American journal of pathology, Vol. 152, Issue 1, pp. 269-78, 1998 (PubMed).

Gonzalez-Zulueta, Ensz, Mukhina, Lebovitz, Zwacka, Engelhardt, Oberley, Dawson, Dawson: "Manganese superoxide dismutase protects nNOS neurons from NMDA and nitric oxide-mediated neurotoxicity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 6, pp. 2040-55, 1998 (PubMed).

Röhrdanz, Kahl: "Alterations of antioxidant enzyme expression in response to hydrogen peroxide." in: Free radical biology & medicine, Vol. 24, Issue 1, pp. 27-38, 1998 (PubMed).