TAP Tag (AA 488-596) antibody

Details for Product No. ABIN968604, Supplier: Log in to see
Antigen
  • Mex67h
  • Tap
  • 1300013M05Rik
  • Spf
  • TAP
  • P115
  • VDP
  • nuclear RNA export factor 1
  • SEC14-like 2 (S. cerevisiae)
  • USO1 vesicle transport factor
  • Nxf1
  • Sec14l2
  • USO1
Epitope
AA 488-596
4
3
1
1
1
1
Reactivity
Tag
18
2
Host
Mouse
17
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
Application
Immunofluorescence (IF), Western Blotting (WB)
20
8
6
4
3
3
2
2
1
1
Options
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Immunogen Human TAP aa. 488-596
Sequence CSSGALDYDI PTTASENLYF Q
Clone 31-TAP
Isotype IgG1
Cross-Reactivity Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Target Type Tag
Background Nuclear export of cellular mRNAs is highly selective, wherein only fully processed RNAs are exported. Retroviral replication requires export of unspliced viral RNAs, which serve as templates for structural proteins and as genomic RNA for progeny virus. The export of unspliced viral RNA depends on the interaction of cis-acting RNA elements with cellular factors. In simian type D retroviruses, the cis-acting element is the constitutive transport element (CTE). CTE-dependent nuclear transport is mediated by the cellular TAP protein. TAP (Tip associating protein) is the vertebrate homolog of the yeast nuclear export protein Mex67p. TAP is a nuclear protein that shuttles between the nucleus and cytoplasm. It contains an N-terminal nuclear localization and export region (NLS-NES), a central RNA binding domain (RBD), and a C-terminal portion that is required for nuclear localization and nuclear rim association. The first 372 aa of TAP are necessary and sufficient for binding and nuclear export of CTE-containing RNA. Thus, TAP contains a novel RNA-binding motif that recognizes CTE-containing RNA and interacts with other components of the nuclear transport machinery.
Molecular Weight 70 kda
Research Area Transporters
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-TAP Tag (AA 488-596) antibody (ABIN968604) Western blot analysis of TAP on Jurkat lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: ...
 image for anti-TAP Tag (AA 488-596) antibody (ABIN968604) Human Endothelial
Product cited in: Bear, Tan, Zolotukhin, Tabernero, Hudson, Felber: "Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs." in: Molecular and cellular biology, Vol. 19, Issue 9, pp. 6306-17, 1999 (PubMed).

Braun, Rohrbach, Schmitt, Izaurralde: "TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus." in: The EMBO journal, Vol. 18, Issue 7, pp. 1953-65, 1999 (PubMed).

Yoon, Lee, Seol, DeMaria, Rosenzweig, Jung: "Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation." in: Immunity, Vol. 6, Issue 5, pp. 571-82, 1997 (PubMed).

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