Casein Kinase II alpha /alpha (AA 1-123) antibody

Catalog No. ABIN968609

Product Details

Target: Casein Kinase II alpha /alpha

Binding Specificity: AA 1-123

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Western Blotting (WB)

Cross-Reactivity: Human, Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Casein Kinase II alpha aa. 1-123

Clone: 31-Casein Kinase IIalpha-alpha'

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968546, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References

Freeman, Brebner, Lynch, Patel, Robertson, Roberts, Vrana: "Changes in rat frontal cortex gene expression following chronic cocaine." in: Brain research. Molecular brain research, Vol. 104, Issue 1, pp. 11-20, (2002) (PubMed).

Greer, Wang, Raman, Justement: "CD45 function is regulated by an acidic 19-amino acid insert in domain II that serves as a binding and phosphoacceptor site for casein kinase 2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 12, pp. 7208-18, (2001) (PubMed).

Xu, Toselli, Russell, Seldin: "Globozoospermia in mice lacking the casein kinase II alpha' catalytic subunit." in: Nature genetics, Vol. 23, Issue 1, pp. 118-21, (1999) (PubMed).

Kikkawa, Mann, Firtel, Hunter: "Molecular cloning of casein kinase II alpha subunit from Dictyostelium discoideum and its expression in the life cycle." in: Molecular and cellular biology, Vol. 12, Issue 12, pp. 5711-23, (1992) (PubMed).

Lozeman, Litchfield, Piening, Takio, Walsh, Krebs: "Isolation and characterization of human cDNA clones encoding the alpha and the alpha' subunits of casein kinase II." in: Biochemistry, Vol. 29, Issue 36, pp. 8436-47, (1991) (PubMed).

Target Details

Target: Casein Kinase II alpha /alpha

Background: Casein kinase II (CKII, CK2) is a pleiotropic, ubiquitous, and constitutively active Ser/Thr kinase that utilizes both ATP and GTP as phosphoryl donors to catalyze the phosphorylation of numerous protein substrates. It has been implicated in DNA replication, control of metabolism, and regulation of transcription and translation. CKII is a messenger independent kinase due to the fact that its activity is not affected by factors that regulate other protein kinases (e.g. cAMP, Ca2+, diacylglycerol). It is composed of two catalytic (alpha and alpha') subunits and two regulatory (beta) subunits. The beta subunits stabilize the holoenzyme and enhance the activity of the alpha subunits. Although the enzyme exists as a tetramer, the free alpha/alpha' subunits are catalytically active by themselves and are present in cells under some conditions. The alpha and alpha' subunits (85% homologous) are encoded by distinct genes and have been detected in multiple species including human, bovine, and avian. CKII is found primarily in the nucleus in dividing cells, but can be detected in the cytoplasm in quiescent cells. Thus, CKII activity is required for cell cycle progression and other essential signal transduction events.

Molecular Weight: 45 kDa