Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF) (AA 1-212) antibody

Antigen: Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF)

Synonyms: PIG7, SIMPLE, TP53I7, FLJ38636, MGC116698, MGC116700, MGC116701, MGC125274, MGC125275, MGC125276

Binding Site: AA 1-212

Clonality: Monoclonal (30)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968613

Additional Information

Alternative name: LITAF

Immunogen: Human LITAF

Format: Liquid

Isotype: IgG1

Clone: 30

Description: TNFalpha (Tumor Necrosis Factor alpha) is a pleiotropic cytokine that conveys either beneficial (prevention of cancers and infection, activation of inflammatory responses) or detrimental (mediation of septic shock, inflammation, and apoptosis) effects to the host. It is produced and secreted primarily by monocytes and macrophages in response to stimulation with lipopolysaccharide (LPS), a principle endotoxic component. LPS-induced TNFalpha factor (LITAF) is a nuclear factor that was purified as a result of its ability to bind to a DNA fragment from the TNFalpha promoter region. LITAF expression is dependent on LPS induction and PMA differentiation in THP-1 cells and is observed at high levels in spleen, lymph node, and peripheral blood leukocytes. Antisense inhibition of LITAF causes a reduction in TNFalpha expression in THP-1 cells. In addition, p53 induces the expression of a transcript called PIG7, which has 98% homology with LITAF. Thus, LITAF is thought to link p53 pathways with the induction of TNFalpha gene expression in response to LPS stimulation and to function, possibly as a transcription factor, to regulate the expression of this highly potent cytokine. This antibody was generated to the LITAF aa 1-212 sequence (Genbank accession # U77396). Recent reports indicate that the nucleotide coding sequence for LITAF may differ and instead, encode for a protein designated as SIMPLE. Under the sequence for SIMPLE, the LITAF aa 1-212 sequence used to generate this antibody corresponds to the aa 1-126 region of SIMPLE. This antibody is routinely tested by western blot analysis.
Synonyms: LPS Induced TNFalpha Factor

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 24 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of LITAF on a A431 cell lysate (Human epithelial carcinoma, ATCC CRL-1555). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti- human LITAF antibody.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).

anti-Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF) (AA 1-212) antibody (Image 3)

Publications

Product: Myokai, Takashiba, Lebo et al.: "A novel lipopolysaccharide-induced transcription factor regulating tumor necrosis factor alpha gene expression: molecular cloning, sequencing, characterization, and chromosomal assignment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4518-23, 1999 (PubMed).

Moriwaki, Begum, Kobayashi et al.: "Mycobacterium bovis Bacillus Calmette-Guerin and its cell wall complex induce a novel lysosomal membrane protein, SIMPLE, that bridges the missing link between lipopolysaccharide and p53-inducible gene, LITAF(PIG7), and estrogen-inducible gene, EET-1." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 23065-76, 2001 (PubMed).

Saifi, Szigeti, Wiszniewski et al.: "SIMPLE mutations in Charcot-Marie-Tooth disease and the potential role of its protein product in protein degradation." in: Human mutation, Vol. 25, Issue 4, pp. 372-83, 2005 (PubMed).