rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 351-453) antibody

Antigen: rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7)

Synonyms: p50, p85, pak3, pixb, cool1, MGC83025, ARHGEF7, P85spr, Pak3bp

Binding Site: AA 351-453

Clonality: Monoclonal (23)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968624

Additional Information

Alternative name: beta PIX

Immunogen: Rat betaPIX

Cross-Reactivity: Chicken, Dog (Canine), Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 23

Description: The activity of PAK family kinases is regulated through interaction with the small GTPases Cdc42 and Rac1. PAKs are activated by the GTP-bound form of Cdc-42 and Rac1, and recruitment of PAKs to focal complexes has been implicated in Cdc42- and Rac1-dependent regulation of focal contact formation. PAK-interacting exchange factor (PIX) was identified in a screen for proteins that bind PAKs. Two forms of PIX have been identified: an 85 kDa protein designated alphaPIX and a 78 kDa protein designated betaPIX. These proteins have 80% identity in their overlapping regions, which include myosin-like, pleckstrin (PH), Dbl (DH), and SH3 domains. In addition, alphaPIX contains a calponin-like domain at the N-terminus. The expression of betaPIX is ubiquitous, while alphaPIX is expressed in heart, muscle, and thymus. PIX can act as a guanine nuleotide exchange factor for Rac1 and co-transfection of betaPIX, Cdc42, and alphaPAK results in increased alphaPAK activity. PIX binding to PAK is required for localization of PAKs to focal complexes and injection of betaPIX leads to Rac1-dependent membrane ruffling. Thus, PIX is important for PAK localization and activity during small GTPase-dependent regulation of cell morphology.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 78 kDa

Comments:

Related Products: ABIN968539, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of betaPIX on PC12 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of betaPIX.

Immunofluorescent staining on MDCK cells.

anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 351-453) antibody (Image 3)

Publications

Product: Oh, Yoo, Jo et al.: "Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion." in: Biochemical and biophysical research communications, Vol. 235, Issue 3, pp. 794-8, 1997 (PubMed).

Manser, Loo, Koh et al.: "PAK kinases are directly coupled to the PIX family of nucleotide exchange factors." in: Molecular cell, Vol. 1, Issue 2, pp. 183-92, 1998 (PubMed).

Bagrodia, Taylor, Jordon et al.: "A novel regulator of p21-activated kinases." in: The Journal of biological chemistry, Vol. 273, Issue 37, pp. 23633-6, 1998 (PubMed).

Turner, Brown, Perrotta et al.: "Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling." in: The Journal of cell biology, Vol. 145, Issue 4, pp. 851-63, 1999 (PubMed).