SLC1A2 antibody (AA 562-574)

Catalog No. ABIN968626

Product Details anti-SLC1A2 Antibody

Target: SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))

Binding Specificity: AA 562-574

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC1A2 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human EAAT2 aa. 562-574

Clone: 20-EAAT2

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-SLC1A2 antibody (ABIN968626)

Grunewald, Kanner: "The accessibility of a novel reentrant loop of the glutamate transporter GLT-1 is restricted by its substrate." in: The Journal of biological chemistry, Vol. 275, Issue 13, pp. 9684-9, (2000) (PubMed).

Yamada, Watanabe, Shibata, Nagashima, Tanaka, Inoue: "Glutamate transporter GLT-1 is transiently localized on growing axons of the mouse spinal cord before establishing astrocytic expression." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 15, pp. 5706-13, (1998) (PubMed).

Tanaka, Watase, Manabe, Yamada, Watanabe, Takahashi, Iwama, Nishikawa, Ichihara, Kikuchi, Okuyama, Kawashima, Hori, Takimoto, Wada: "Epilepsy and exacerbation of brain injury in mice lacking the glutamate transporter GLT-1." in: Science (New York, N.Y.), Vol. 276, Issue 5319, pp. 1699-702, (1997) (PubMed).

Kirschner, Copeland, Gilbert, Jenkins, Amara: "Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2." in: Genomics, Vol. 24, Issue 2, pp. 218-24, (1995) (PubMed).

Target Details for SLC1A2

Target: SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))

Alternative Name: EAAT2 (SLC1A2 Products)

Synonyms: CG3159 antibody, Dmel\\CG3159 antibody, EAAT antibody, dEAAT2 antibody, dEaat2 antibody, Eaat antibody, GB16377 antibody, EAAT2 antibody, SLC1A2 antibody, Eaat2 antibody, Glt antibody, Glt-1 antibody, GLT-1 antibody, 1700091C19Rik antibody, 2900019G14Rik antibody, AI159670 antibody, GLT1 antibody, MGLT1 antibody, eaat2 antibody, fj34b12 antibody, slc1a2 antibody, wu:fj34b12 antibody, zgc:65897 antibody, Excitatory amino acid transporter 2 antibody, excitatory amino acid transporter 2 antibody, solute carrier family 1 member 2 antibody, solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody, solute carrier family 1 (glial high affinity glutamate transporter), member 2b antibody, Eaat2 antibody, Eaat-2 antibody, SLC1A2 antibody, VDBG_06180 antibody, EUBELI_01747 antibody, Trebr_0139 antibody, Slc1a2 antibody, slc1a2b antibody

Background: Electrogenic-coupled (Na+ and K+) glutamate transporters in nerve terminals and glial cells are essential for maintaining subtoxic levels of extracellular excitatory amino acid neurotransmitters (e.g. glutamate and aspartate). They are also important for modifying synaptic transmission through the cotransport of ions and neurotransmitters. Excitatory amino acid transporter 2 (EAAT2) has 8 alpha-helical transmembrane domains and two reentrant pore-loop-like structures whose conformation is altered during ion neurotransmitter transport. EAAT2 is primarily expressed in astrocytes in adult brain and spinal cord, however it is also found in axons during fetal development. Mice deficient in EAAT2 exhibit lethal epileptic seizures with destruction of hippocampal neurons and an increased susceptibility to cold-induced cortical injury. Such defects in glutamine transporters have also been implicated in stroke, brain trauma, Alzheimer's disease, amyotrophic lateral sclerosis, and Huntington's disease. Thus, glutamine transporters, such as EAAT2, are critical for prevention of neurotoxic brain injury and may regulate glutamatergic synaptic transmission. This antibody is routinely tested by western blot analysis.
Synonyms: Excitatory amino acid transporter 2, Glutamate transporter 1, GLT1

Molecular Weight: 66 kDa

Pathways: Dicarboxylic Acid Transport