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Espin antibody (AA 458-580)

ESPN Reactivity: Human, Mouse, Rat, Chicken WB, IF Host: Mouse Monoclonal 31-Espin unconjugated
Catalog No. ABIN968627
  • Target See all Espin (ESPN) Antibodies
    Espin (ESPN)
    Binding Specificity
    • 8
    • 8
    • 1
    AA 458-580
    Reactivity
    Human, Mouse, Rat, Chicken
    Host
    • 26
    Mouse
    Clonality
    • 26
    Monoclonal
    Conjugate
    • 9
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Espin antibody is un-conjugated
    Application
    • 18
    • 15
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Human, Mouse (Murine), Chicken
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Rat espin aa. 458-580
    Clone
    31-Espin
    Isotype
    IgG2a
    Top Product
    Discover our top product ESPN Primary Antibody
  • Comment

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Chen, Li, Wang, Wang, Zheng, Bartles: "Espin contains an additional actin-binding site in its N terminus and is a major actin-bundling protein of the Sertoli cell-spermatid ectoplasmic specialization junctional plaque." in: Molecular biology of the cell, Vol. 10, Issue 12, pp. 4327-39, (2000) (PubMed).

    Bartles, Zheng, Li, Wierda, Chen: "Small espin: a third actin-bundling protein and potential forked protein ortholog in brush border microvilli." in: The Journal of cell biology, Vol. 143, Issue 1, pp. 107-19, (1998) (PubMed).

    Bartles, Wierda, Zheng: "Identification and characterization of espin, an actin-binding protein localized to the F-actin-rich junctional plaques of Sertoli cell ectoplasmic specializations." in: Journal of cell science, Vol. 109 ( Pt 6), pp. 1229-39, (1997) (PubMed).

  • Target
    Espin (ESPN)
    Alternative Name
    Espin (ESPN Products)
    Synonyms
    DFNB36 antibody, je antibody, Je antibody, espin antibody, ESPN antibody, TVAG_100530 antibody, LOAG_01035 antibody, espn antibody, Espn antibody
    Background
    Actin-binding proteins regulate the polymerization and depolymerization of actin, connect actin-based structures to membranes and to other cytoskeletal elements, power the movement of actin filaments, and cross-link actin filaments into bundles. Espins are actin binding and bundling proteins. The two isoforms of espin are the 30 kDa small espin found in brush border cells and the 110 kDa espin found in testis. Espin contains eight ankyrin repeats in the N-terminal region, two proline-rich peptides, an ATP/GTP binding P-loop domain, and a C-terminal actin bundling domain. Small espin is composed of the C-terminal actin bundling domain and a unique region at the N-terminus. Espin binds to actin with a higher affinity than small espin and is more efficient at actin bundling. During spermiogenesis, espin accumulates, along with forming parallel actin bundles, at the ectoplasmic specialization. These actin bundles anchor and position the spermatid within the seminiferous epithelium. Other actin binding proteins, such as alpha-actinin, vinculin, and fimbrin, have also been implicated in the formation of ectoplasmic specialization, however espin appears to function specifically in the testes.
    Molecular Weight
    110 kDa
    Pathways
    Sensory Perception of Sound
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