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PSIP1 antibody (AA 85-188)

PSIP1 Reactivity: Human WB, IF, BI Host: Mouse Monoclonal 26-LEDGF unconjugated
Catalog No. ABIN968640
  • Target See all PSIP1 Antibodies
    PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))
    Binding Specificity
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 85-188
    Reactivity
    • 26
    • 19
    • 12
    • 12
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    Human
    Host
    • 16
    • 13
    Mouse
    Clonality
    • 16
    • 13
    Monoclonal
    Conjugate
    • 22
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PSIP1 antibody is un-conjugated
    Application
    • 19
    • 9
    • 8
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human LEDGF aa. 85-188
    Clone
    26-LEDGF
    Isotype
    IgG1
    Top Product
    Discover our top product PSIP1 Primary Antibody
  • Application Notes
    Bioimaging:
    Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
    Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
    Comment

    Related Products: ABIN968535, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Cherepanov, Maertens, Proost, Devreese, Van Beeumen, Engelborghs, De Clercq, Debyser: "HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells." in: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 372-81, (2002) (PubMed).

    Nakamura, Singh, Kubo, Chylack, Shinohara: "LEDGF: survival of embryonic chick retinal photoreceptor cells." in: Investigative ophthalmology & visual science, Vol. 41, Issue 5, pp. 1168-75, (2000) (PubMed).

    Singh, Kimura, Chylack, Shinohara: "Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicing." in: Gene, Vol. 242, Issue 1-2, pp. 265-73, (2000) (PubMed).

    Ge, Si, Wolffe: "A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2." in: Molecular cell, Vol. 2, Issue 6, pp. 751-9, (1999) (PubMed).

    Ge, Si, Roeder: "Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation." in: The EMBO journal, Vol. 17, Issue 22, pp. 6723-9, (1999) (PubMed).

  • Target
    PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))
    Alternative Name
    LEDGF (PSIP1 Products)
    Synonyms
    PSIP1 antibody, im:7149861 antibody, zgc:109898 antibody, LEDGF antibody, p75 antibody, PSIP2 antibody, DFS70 antibody, PAIP antibody, p52 antibody, AA408851 antibody, AU015605 antibody, Dfs70 antibody, Ledgfa antibody, Ledgfb antibody, Psip2 antibody, Ledgf antibody, SBP75 antibody, LEDGF/p75 antibody, PC4 and SFRS1 interacting protein 1 S homeolog antibody, PC4 and SFRS1 interacting protein 1 antibody, PC4 and SFRS1 interacting protein 1a antibody, LEDGF/p75 transcription factor-like antibody, psip1.S antibody, PSIP1 antibody, psip1a antibody, psip1 antibody, Psip1 antibody
    Background
    P52/p75 proteins were first identified through their interaction with transcriptional factors and splicing factors. p75 is encoded by exons 1-15 and contains 530 amino acids, while the alternatively spliced p52 is encoded by exons 1-9 and contains 333 amino acids. Both p52 and p75 have strong interactions with the VP16 activation domain and with various components of the general transcriptional machinery. p52 is the more active transcription activator and has also been shown to recruit the pre-mRNA splicing factor ASF/SF to sites of active transcription. Interestingly, p52/p75 has also been identified as Lens Epithelium-Derived Growth Factor (LEDGF). In serum free media, LEDGF stimulates the growth and survival of a variety of cells, such as lens epithelial cells, retinal photoreceptor cells, COS7 cells, skin fibroblasts, and keratinocytes. In retinal photoreceptor cells, LEDGF is found in the nucleus where it promotes resistance to serum starvation and thermal stress. Thus, the LEDGF gene encodes splice variants that may regulate gene transcription and mRNA splicing of a variety of proteins that are essential for cell growth and survival.
    Synonyms: Lens Epithelium-Derived Growth Factor
    Molecular Weight
    75 kDa and/or 52 kDa
    Pathways
    Chromatin Binding, Ribonucleoprotein Complex Subunit Organization
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