Immunofluorescent staining of A549 (ATCC CCL-185) cells. Cells were seeded in a 96 well imaging plate at ~10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the Mouse Anti-Bub3 antibody. The second step reagent was FITC goat anti mouse Ig. The image was taken on a BD Pathway™ 855 bioimaging system using a 20x objective. This antibody also stains HeLa (ATCC CCL-2) and U-2 OS (ATCC HTB-96) cells and can be used with either fix/perm protocol.