CaM Kinase VI (AA 330-424) antibody
Western Blotting (WB), Immunofluorescence (IF)
|3 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
Central nervous system plasticity is essential to the processes of learning and memory. Plasticity involves changes in synaptic transmission efficiency and results from the activation of excitatory glutamate receptors. Analysis of changes in gene expression during kainate-induced hippocampal plasticity led to the identification of a Candidate Plasticity-related Gene (CPG16). CPG16 protein is a Ser/Thr protein kinase that is also closely related to the Ca2+/calmodulin- dependent protein kinases (CaM kinases). CPG16 is 98% identical to the C-terminal region of the product of the KIAA0369 gene, a 96 kDa protein whose N-terminal region strongly resembles the neural protein, doublecortin. In resting cells, Cam Kinase VI (CPG16) is found in the cytoplasm and has relatively low level of phosphorylation activity. However, treatment of cells with cAMP-elevating agents (forskolin, 8-bromo-cAMP) significantly enhances this phosphorylation activity and induces a partial shift of Cam Kinase VI (CPG16) into the nucleus. In addition, overexpression of Cam Kinase VI (CPG16) inhibits cAMP-stimulated CREB activity. Thus, Cam Kinase VI (CPG16) is Ser/Thr protein kinase that is thought to be a downstream negative regulator of cAMP/PKA-induced transcription.
Synonyms: CPG16, Candidate Plasticity-related Gene 16
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||96 & 48 kDa|
Related Products: ABIN968545, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
Gleeson, Allen, Fox et al.: "Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein." in: Cell, Vol. 92, Issue 1, pp. 63-72, 1998 (PubMed).
Hevroni, Rattner, Bundman et al.: "Hippocampal plasticity involves extensive gene induction and multiple cellular mechanisms." in: Journal of molecular neuroscience : MN, Vol. 10, Issue 2, pp. 75-98, 1998 (PubMed).
Silverman, Benard, Jaaro et al.: "CPG16, a novel protein serine/threonine kinase downstream of cAMP-dependent protein kinase." in: The Journal of biological chemistry, Vol. 274, Issue 5, pp. 2631-6, 1999 (PubMed).