CaM Kinase VI (AA 330-424) antibody

Antigen: CaM Kinase VI

Binding Site: AA 330-424

Clonality: Monoclonal (5)

Host: Mouse

Reactivity: Rat (Rattus)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968650

Additional Information

Immunogen: Rat CPG16

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 5

Description: Central nervous system plasticity is essential to the processes of learning and memory. Plasticity involves changes in synaptic transmission efficiency and results from the activation of excitatory glutamate receptors. Analysis of changes in gene expression during kainate-induced hippocampal plasticity led to the identification of a Candidate Plasticity-related Gene (CPG16). CPG16 protein is a Ser/Thr protein kinase that is also closely related to the Ca2+/calmodulin- dependent protein kinases (CaM kinases). CPG16 is 98% identical to the C-terminal region of the product of the KIAA0369 gene, a 96 kDa protein whose N-terminal region strongly resembles the neural protein, doublecortin. In resting cells, Cam Kinase VI (CPG16) is found in the cytoplasm and has relatively low level of phosphorylation activity. However, treatment of cells with cAMP-elevating agents (forskolin, 8-bromo-cAMP) significantly enhances this phosphorylation activity and induces a partial shift of Cam Kinase VI (CPG16) into the nucleus. In addition, overexpression of Cam Kinase VI (CPG16) inhibits cAMP-stimulated CREB activity. Thus, Cam Kinase VI (CPG16) is Ser/Thr protein kinase that is thought to be a downstream negative regulator of cAMP/PKA-induced transcription.
Synonyms: CPG16, Candidate Plasticity-related Gene 16

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 96 & 48 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of CaM Kinase VI on a rat cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-CaM Kinase VI antibody. CaM Kinase VI (CPG16) has been reported to be a splice variant of DCAMKL1 (Doublecortin-like and CAM kinase-like 1). The immunogen used to generate this antibody has been reported to be shared between the two variants with CaM Kinase VI (CPG16) being detectable at 48 kD.

Immunofluorescence staining of cortical neurons.

Publications

Product: Gleeson, Allen, Fox et al.: "Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein." in: Cell, Vol. 92, Issue 1, pp. 63-72, 1998 (PubMed).

Hevroni, Rattner, Bundman et al.: "Hippocampal plasticity involves extensive gene induction and multiple cellular mechanisms." in: Journal of molecular neuroscience : MN, Vol. 10, Issue 2, pp. 75-98, 1998 (PubMed).

Silverman, Benard, Jaaro et al.: "CPG16, a novel protein serine/threonine kinase downstream of cAMP-dependent protein kinase." in: The Journal of biological chemistry, Vol. 274, Issue 5, pp. 2631-6, 1999 (PubMed).