SLC9A1 antibody (AA 682-801)

Catalog No. ABIN968659

Product Details anti-SLC9A1 Antibody

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Binding Specificity: AA 682-801

Reactivity: Human, Rat, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC9A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse (Murine), Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat NHE-1 aa. 682-801

Clone: 54-NHE

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-SLC9A1 antibody (ABIN968659)

Zhao, Oxley, Smith, Follows, Green, Alexander: "DNA damage-induced Bcl-xL deamidation is mediated by NHE-1 antiport regulated intracellular pH." in: PLoS biology, Vol. 5, Issue 1, pp. e1, (2007) (PubMed).

Aharonovitz, Zaun, Balla, York, Orlowski, Grinstein: "Intracellular pH regulation by Na(+)/H(+) exchange requires phosphatidylinositol 4,5-bisphosphate." in: The Journal of cell biology, Vol. 150, Issue 1, pp. 213-24, (2000) (PubMed).

Cox, Lutz, Yang, Biemesderfer, Bronson, Fu, Aronson, Noebels, Frankel: "Sodium/hydrogen exchanger gene defect in slow-wave epilepsy mutant mice." in: Cell, Vol. 91, Issue 1, pp. 139-48, (1997) (PubMed).

Kandasamy, Yu, Harris, Boucher, Hanrahan, Orlowski: "Plasma membrane Na+/H+ exchanger isoforms (NHE-1, -2, and -3) are differentially responsive to second messenger agonists of the protein kinase A and C pathways." in: The Journal of biological chemistry, Vol. 270, Issue 49, pp. 29209-16, (1996) (PubMed).

Orlowski, Kandasamy, Shull et al.: "Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally ..." in: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9331-9, (1992) (PubMed).

Target Details for SLC9A1

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Alternative Name: NHE-1 (SLC9A1 Products)

Synonyms: AT-NHX1 antibody, ATNHX antibody, ATNHX1 antibody, NA(+)/H(+) ANTIPORTER antibody, Na+/H+ exchanger 1 antibody, T21B4.60 antibody, T21B4_60 antibody, AW554487 antibody, Apnh antibody, Nhe1 antibody, swe antibody, NHE-1 antibody, NHE1 antibody, APNH antibody, Na+/H+ exchanger 1 antibody, solute carrier family 9 (sodium/hydrogen exchanger), member 1 antibody, solute carrier family 9 member A1 antibody, NHX1 antibody, Slc9a1 antibody, SLC9A1 antibody

Background: The extrusion of H+ in exchange for extracellular Na+ is important for many cellular processes, such as pH homeostasis, volume regulation, and transepithelial ion and water transport. Na+/H+ Exchangers (NHE) are integral membrane proteins that mediate electroneutral exchange of one Na+ ion for one H+ ion. Six NHE forms, NHE-1 thru -6, have been identified. NHE-1 and NHE-6 are widely expressed, while the other NHE forms have restricted expression. The common structure of all NHE forms includes 10-12 N-terminal membrane (M) spanning regions, a conserved M6 and M7 region that may participate in ion transport, and a large C-terminal cytoplasmic region that may be involved in the regulation of ion exchange activity. NHE-1, for example, contains 12 M regions plus domains for volume sensitivity, calmodulin-binding, CHP-binding, and PKC phosphorylation in the cytoplasmic region. Regulation of NHE-1 ion exchange activity may occur through phosphoinositide binding, as well as PKC- and PKA-dependent signaling pathways. Mutation of NHE-1 in mice causes neuronal death in the cerebellum and brainstem, leading to ataxia and seizures. Thus, NHE-1 is a ubiquitous NHE that is essential for normal brain function.
Although this antibody was developed against the NHE-1 antigen, investigators should note that crossreactivity to other NHE isoforms or variants may be possible.
Synonyms: Na+/H+ Exchangers

Molecular Weight: 92 kDa

Pathways: Glycosaminoglycan Metabolic Process, Proton Transport