Integrin-Linked Kinase (ILK) (AA 326-452) antibody
|Synonyms||ESTM24, AA511515, P59, DKFZp686F1765, CT29478, DmILK, ILK, l(3)78Ca, DmelCG10504, CG10504, MGC65842, zgc:65842, ilk, MGC82501, MGC130696, MGC129022, Ilk, p59|
Alternatives Western Blotting (WB)
|5 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)|
|Description||Integrins are transmembrane receptors that mediate cell-cell or cell-matrix adhesion. All integrins are heterodimers composed of alpha and beta subunits, which interact with extracellular matrix and cytoskeletal proteins. Signal transduction through intergin receptors may be regulated by integrin-linked kinase (ILK). ILK is a widely expressed Ser/Thr protein kinase that contains four ankyrin-like repeats in the N-terminal region, a phoshoinositide lipid-binding motif at amino acids 180-212, and an integrin binding site at amino acids 293-451. The ankyrin repeats interact with a LIM domain-only protein called PINCH that also binds the adaptor protein, Nck-2. This interaction implicates ILK in growth factor receptor pathways. In addition, the interaction of ILK with the cytoplasmic domains of integrin beta1 and beta3 subunits and localization of ILK to focal adhesion plaques suggests a role for ILK in the regulation of cell adhesion. Interestingly, overexpression of ILK in epithelial cells inhibits cell adhesion and stimulates cell cycle progression and tumorigenicity. Thus, ILK regulates signal transduction in many different cell signaling pathways. This antibody is routinely tested by western blot analysis.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||50 kDa|
Related Products: ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
|Western blot analysis of ILK on Hs68 cell lysate Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-ILK antibody.|
Hannigan, Leung-Hagesteijn, Fitz-Gibbon et al.: "Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase." in: Nature, Vol. 379, Issue 6560, pp. 91-6, 1996 (PubMed).
Delcommenne, Tan, Gray et al.: "Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 19, pp. 11211-6, 1998 (PubMed).
Persad, Attwell, Gray et al.: "Inhibition of integrin-linked kinase (ILK) suppresses activation of protein kinase B/Akt and induces cell cycle arrest and apoptosis of PTEN-mutant prostate cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3207-12, 2000 (PubMed).
Nikolopoulos, Turner: "Integrin-linked kinase (ILK) binding to paxillin LD1 motif regulates ILK localization to focal adhesions." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23499-505, 2001 (PubMed).
Nikolopoulos, Turner: "Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization." in: The Journal of biological chemistry, Vol. 277, Issue 2, pp. 1568-75, 2002 (PubMed).