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Integrin-Linked Kinase (ILK) (AA 326-452) antibody

Details for Product No. ABIN968662, Supplier: Log in to see
Antigen
  • CG10504
  • CT29478
  • DmILK
  • Dmel\\CG10504
  • ILK
  • ilk
  • l(3)78Ca
  • p59
  • AA511515
  • ESTM24
  • zgc:65842
  • ILK-1
  • ILK-2
  • P59
  • p59ILK
  • Integrin linked kinase
  • integrin-linked kinase
  • integrin-linked protein kinase
  • Integrin-linked protein kinase
  • integrin linked kinase
  • Ilk
  • ILK
  • ilk
  • CpipJ_CPIJ016644
  • Bm1_20815
  • Tsp_07213
  • Tsp_07224
  • Tsp_15354
Alternatives
anti-Human Integrin-Linked Kinase antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 326-452
37
28
24
15
15
15
13
12
10
8
7
7
7
4
4
4
4
3
3
3
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
251
101
90
5
4
3
3
2
2
2
1
1
1
1
Host
Mouse
226
14
12
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
18
17
17
11
11
11
3
3
3
3
3
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
149
134
100
39
25
22
13
6
5
4
3
3
1
Options
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Immunogen Human ILK aa. 326-452
Clone 3-ILK
Isotype IgG1
Cross-Reactivity Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name ILK (ILK Antibody Abstract)
Background Integrins are transmembrane receptors that mediate cell-cell or cell-matrix adhesion. All integrins are heterodimers composed of alpha and beta subunits, which interact with extracellular matrix and cytoskeletal proteins. Signal transduction through intergin receptors may be regulated by integrin-linked kinase (ILK). ILK is a widely expressed Ser/Thr protein kinase that contains four ankyrin-like repeats in the N-terminal region, a phoshoinositide lipid-binding motif at amino acids 180-212, and an integrin binding site at amino acids 293-451. The ankyrin repeats interact with a LIM domain-only protein called PINCH that also binds the adaptor protein, Nck-2. This interaction implicates ILK in growth factor receptor pathways. In addition, the interaction of ILK with the cytoplasmic domains of integrin beta1 and beta3 subunits and localization of ILK to focal adhesion plaques suggests a role for ILK in the regulation of cell adhesion. Interestingly, overexpression of ILK in epithelial cells inhibits cell adhesion and stimulates cell cycle progression and tumorigenicity. Thus, ILK regulates signal transduction in many different cell signaling pathways. This antibody is routinely tested by western blot analysis.
Molecular Weight 50 kDa
Pathways Regulation of Muscle Cell Differentiation
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Integrin-Linked Kinase (ILK) (AA 326-452) antibody (ABIN968662) Western blot analysis of ILK on Hs68 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lan...
Product cited in: Nikolopoulos, Turner: "Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization." in: The Journal of biological chemistry, Vol. 277, Issue 2, pp. 1568-75, 2002 (PubMed).

Nikolopoulos, Turner: "Integrin-linked kinase (ILK) binding to paxillin LD1 motif regulates ILK localization to focal adhesions." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23499-505, 2001 (PubMed).

Persad, Attwell, Gray, Delcommenne, Troussard, Sanghera, Dedhar: "Inhibition of integrin-linked kinase (ILK) suppresses activation of protein kinase B/Akt and induces cell cycle arrest and apoptosis of PTEN-mutant prostate cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3207-12, 2000 (PubMed).

Delcommenne, Tan, Gray, Rue, Woodgett, Dedhar: "Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 19, pp. 11211-6, 1998 (PubMed).

Hannigan, Leung-Hagesteijn, Fitz-Gibbon, Coppolino, Radeva, Filmus, Bell, Dedhar: "Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase." in: Nature, Vol. 379, Issue 6560, pp. 91-6, 1996 (PubMed).