Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr694) antibody

Details for Product No. ABIN968670
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Synonyms STAT5A, STAT5, MGF, AA959963, Stat5, STAT5A/MGF, STATA5
(59), (42), (39), (26), (16), (12), (10), (6), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(335), (169), (138), (15), (3), (2), (1), (1)
(302), (55), (8)
(10), (9), (8), (7), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Western Blotting (WB), Immunofluorescence (IF)
(315), (164), (130), (76), (38), (38), (34), (31), (25), (17), (13), (10), (8), (6), (5), (3), (2), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 150 μg
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Catalog No. ABIN968670
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Isotype Ig
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from antiserum by negative adsorption and affinity chromatography.
Alternative Name Stat5
Background Mammary gland factor (MGF) has been shown to play a critical role in the lactogenic hormone response and has been well characterized in mammary epithelial cells. The SH3 domain of MGF shows extensive homology with the SH2 domains of Stat1 and Stat2. This homology leads to speculation that MGF may have a broader physiological role than originally anticipated and has led to its classification as a member of the Stat family. Stat5 (MGF) mRNA expression is highest in mammary gland tissue. Lower levels are found in ovary, thymus, spleen, kidney, lung, muscle, and the adrenal gland. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92 kDa Stat5 protein by inducing phosphorylation at Tyr694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. Stat5 activity is tightly regulated throughout gestation,lactation, and post-lactation. Treatment of activated Stat5 with a protein tyrosine phosphatase results in the loss of DNA binding activity. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. The immunogen used to generate this antibody was a phosphorylated sheep stat5 peptide.
Molecular Weight 92 kDa
Research Area Signaling, Receptors, Inflammation

Related Products: ABIN967390

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr694) antibody Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2.2).
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr694) antibody (2) U-937 cells (Human histiocytic lymphoma, ATCC CRL-1593.2) were either untreated (lane 1) or treated with 15 ng/ml with GM-CSF for 20 minutes at 37°C (lane 2). The top panel, representing total stat5, was probed with purified mouse anti-stat5 (ABIN967810) and tbe bottom panel, representing phosphorylated stat5 (pY694), was probed with the polyclonal anti-human stat 5 (pY694) antibody at a 1:250 dilution.
Product cited in: Gouilleux, Wakao, Mundt et al.: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, 1994 (PubMed).

Gouilleux, Pallard, Dusanter-Fourt et al.: "Prolactin, growth hormone, erythropoietin and granulocyte-macrophage colony stimulating factor induce MGF-Stat5 DNA binding activity." in: The EMBO journal, Vol. 14, Issue 9, pp. 2005-13, 1995 (PubMed).

Wakao, Gouilleux, Groner: "Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response." in: The EMBO journal, Vol. 13, Issue 9, pp. 2182-91, 1994 (PubMed).

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