Kallikrein 10 (KLK10) (AA 99-202) antibody

Antigen: Kallikrein 10 (KLK10)

Synonyms: ACO, HSRNASPH, NES1, PRSSL1, TLSP, PRSS20, MGC33060, KLK-L6, 2300002A13Rik, KLK10

Binding Site: AA 99-202

Clonality: Monoclonal (2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968673

Additional Information

Alternative name: NES1

Immunogen: Human NES1

Format: Liquid

Isotype: IgG1

Clone: 2

Description: Normal epithelial cell-specific 1 (NES1) was identified in a screen of genes whose mRNA is down-regulated during oncogenic transformation of human mammary epithelial cells (MECs). The protein is homologous to multiple serine proteases including members of the trypsin and kallikrein protease families. Similar to other serine proteases, the sequence of NES1 contains three residues (Ser229, Asp137, and His86) that form a catalytic triad and a putative cleavage site (Arg42). The pattern of NES1 expression in normal human tissues includes prostate, testis, ovary, small intestine, colon, and lung. In addition, expression has been reported to be reduced in human breast and prostate cancer cell lines. NES1 is a secreted protein found in the supernantant of growing MECs. Expression of NES1 in a breast cancer cell line has been reported to suppress oncogenic cell behavior, such as anchorage-independent growth and tumor formation in nude mice. In the breast cancer cell line BT-474, NES1 levels reportedly are up-regulated by steroid hormones, such as estrogen, androgen, and progestin. Thus, NES1 is a steroid-hormone regulated, serine protease that is thought to have important tumor suppressor functions. This antibody is routinely tested by western blot analysis.
Synonyms: Normal Epithelial cell Specific-1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 30 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of NES1 on a NIH:OVCAR3 cell lysate (Human ovary epithelial adenocarcinoma, ATCC HTB-161). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human NES1 antibody.

Publications

Product: Liu, Wazer, Watanabe et al.: "Identification of a novel serine protease-like gene, the expression of which is down-regulated during breast cancer progression." in: Cancer research, Vol. 56, Issue 14, pp. 3371-9, 1996 (PubMed).

Goyal, Smith, Cowan et al.: "The role for NES1 serine protease as a novel tumor suppressor." in: Cancer research, Vol. 58, Issue 21, pp. 4782-6, 1998 (PubMed).

Luo, Grass, Diamandis: "The normal epithelial cell-specific 1 (NES1) gene is up-regulated by steroid hormones in the breast carcinoma cell line BT-474." in: Anticancer research, Vol. 20, Issue 2A, pp. 981-6, 2000 (PubMed).