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Chromodomain Helicase DNA Binding Protein 3 (CHD3) (AA 1277-1474) antibody

Details for Product No. ABIN968677, Supplier: Login to see
Antigen
  • CG9594
  • CHD-3
  • CHD3
  • DmCHD3
  • Dmel\\CG9594
  • chd3
  • dCHD3
  • LOC568230
  • zfh
  • mi-2a
  • mi2-alpha
  • Mi-2a
  • Mi2-ALPHA
  • ZFH
  • 2600010P09Rik
  • AF020312
  • Chd7
  • Prp7
  • Prp9-1
Epitope
AA 1277-1474
7
5
5
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
59
29
16
2
1
1
Host
Mouse
33
32
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
43
25
18
17
15
13
13
11
6
3
2
1
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Immunogen Human CHD3 aa. 1277-1474
Clone 44-CHD3
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CHD3 (CHD3 Antibody Abstract)
Background The SNF2-related family of proteins all contain conserved helicase motifs, and have been implicated in transcription regulation, DNA repair, and DNA recombination. The DNA-dependent ATPase activity of these proteins is usually activated by single-stranded, double-stranded, or nucleosomal DNA. The chromodomain helicase DNA binding proteins (CHDs) are a subfamily of the SNF2 family, and includes CHD1, CHD2, CHD3, and CHD4. CHD3 and CHD4 contain N-terminal paired PHD zinc finger motifs (PZF), two heterochromatin binding domains (chromodomains, CDs), and a central SNF2-like helicase/ATPase domain. CHD3 mRNA is widely expressed, and CHD3 protein localizes to the nucleus, but not nucleoli. Both CHD3 and CHD4 were first identified as self-antigens recognized by sera from patients with the autoimmune connective-tissue disease Mi-2 dermatomyositis. In addition, both CHD3 and CHD4 are associated with a nucleosome remodelling and deacetylating (NRD) complex, and facilitate deacetylation of oligonucleosomal histones in vitro. Thus, CHD3 and CHD4 are chromodomain containing proteins that may have important roles in ATP-dependent nucleosome remodelling.
Molecular Weight 221 kDa
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) (AA 1277-1474) antibody (ABIN968677) Western blot analysis of CHD3 on human endothelial cell lysate. Lane 1: 1:250, lane 2...
Immunofluorescence (IF) image for anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) (AA 1277-1474) antibody (ABIN968677) Immunofluorescent staining of human endothelial cells
Product cited in: Woodage, Basrai, Baxevanis et al.: "Characterization of the CHD family of proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 21, pp. 11472-7, 1997 (PubMed).

Background publications Tong, Hassig, Schnitzler et al.: "Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex." in: Nature, Vol. 395, Issue 6705, pp. 917-21, 1998 (PubMed).