Chromodomain Helicase DNA Binding Protein 3 (CHD3) (AA 1277-1474) antibody

Antigen: Chromodomain Helicase DNA Binding Protein 3 (CHD3)

Synonyms: ZFH, Mi-2a, Mi2-ALPHA, Chd7, Prp7, Prp9-1, AF020312, MGC40857, 2600010P09Rik, CHD3, LOC568230, zfh, mi-2a, mi2-alpha

Binding Site: AA 1277-1474

Clonality: Monoclonal (44)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968678

Additional Information

Alternative name: CHD3

Immunogen: Human CHD3

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 44

Description: The SNF2-related family of proteins all contain conserved helicase motifs, and have been implicated in transcription regulation, DNA repair, and DNA recombination. The DNA-dependent ATPase activity of these proteins is usually activated by single-stranded, double-stranded, or nucleosomal DNA. The chromodomain helicase DNA binding proteins (CHDs) are a subfamily of the SNF2 family, and includes CHD1, CHD2, CHD3, and CHD4. CHD3 and CHD4 contain N-terminal paired PHD zinc finger motifs (PZF), two heterochromatin binding domains (chromodomains, CDs), and a central SNF2-like helicase/ATPase domain. CHD3 mRNA is widely expressed, and CHD3 protein localizes to the nucleus, but not nucleoli. Both CHD3 and CHD4 were first identified as self-antigens recognized by sera from patients with the autoimmune connective-tissue disease Mi-2 dermatomyositis. In addition, both CHD3 and CHD4 are associated with a nucleosome remodelling and deacetylating (NRD) complex, and facilitate deacetylation of oligonucleosomal histones in vitro. Thus, CHD3 and CHD4 are chromodomain containing proteins that may have important roles in ATP-dependent nucleosome remodelling.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 221 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of CHD3 on human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-CHD3.

Immunofluorescent staining of human endothelial cells

Publications

Product: Woodage, Basrai, Baxevanis et al.: "Characterization of the CHD family of proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 21, pp. 11472-7, 1997 (PubMed).

Tong, Hassig, Schnitzler et al.: "Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex." in: Nature, Vol. 395, Issue 6705, pp. 917-21, 1998 (PubMed).