NOS1 antibody (Nitric Oxide Synthase 1, Neuronal) (AA 144-262)

Details for Product anti-NOS1 Antibody No. ABIN968680, Supplier: Log in to see
Antigen
  • nos
  • nNOS
  • NOS1
  • IHPS1
  • N-NOS
  • NC-NOS
  • NOS
  • bNOS
  • 2310005C01Rik
  • NO
  • NOS-I
  • Nos-1
  • nitric oxide synthase 1 (neuronal)
  • nitric oxide synthase
  • nitric oxide synthase 1, neuronal
  • nos1
  • NOS1
  • NNOS
  • Nos1
Alternatives
anti-Human NOS1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 144-262
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Reactivity
Rat (Rattus)
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This NOS1 antibody is un-conjugated
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Application
Immunofluorescence (IF), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen Rat nNOS/NOS Type I aa. 144-262
Clone 52-nNOS-NOS Type I
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name nNOS/NOS Type I (NOS1 Antibody Abstract)
Background Nitric oxide synthase (NOS) is a cell-type specific enzyme which catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical which transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+concentrations and enhance calmodulin binding. Neuronal NOS (nNOS or bNOS) and endothelial NOS (eNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and are regulated in a similar manner. However, both have been shown to be distinct gene products of about 155kDa and 140kDa, respectively, and the human forms show 52% amino acid identity to each other. nNOS and the 130 kDa inducible macrophage NOS (iNOS) share 51% amino acid homology. nNOS (neuronal NOS) is found in the cytoplasm of cell types such as neurons, skeletal muscle fibers, and lung epithelium. It is slightly larger than the other isoforms due to an N-terminal extension containing a PDZ domain through which it binds PSD-95 (post-synaptic density protein) and localizes to the cell membrane of synapses.
Molecular Weight 155 kDa
Pathways Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 144-262) antibody (ABIN968680) Western blot analysis of nNOS/NOS Type I on rat pituitary lysate. Lane 1: 1:2500, lan...
 image for anti-Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 144-262) antibody (ABIN968680) GC
Product cited in: Tochio, Mok, Zhang, Kan, Bredt, Zhang: "Formation of nNOS/PSD-95 PDZ dimer requires a preformed beta-finger structure from the nNOS PDZ domain." in: Journal of molecular biology, Vol. 303, Issue 3, pp. 359-70, 2000 (PubMed).

Nathan: "Nitric oxide as a secretory product of mammalian cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 6, Issue 12, pp. 3051-64, 1992 (PubMed).

Background publications Bredt, Hwang, Glatt, Lowenstein, Reed, Snyder: "Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase." in: Nature, Vol. 351, Issue 6329, pp. 714-8, 1991 (PubMed).

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