NEDD8 Activating Enzyme E1 Subunit 1 (NAE1) (AA 348-464) antibody

Antigen: NEDD8 Activating Enzyme E1 Subunit 1 (NAE1)

Synonyms: APPBP1, anon-WO0140519.225, DmelCG7828

Binding Site: AA 348-464

Clonality: Monoclonal (20)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN968684

Additional Information

Alternative name: APP-BP1

Immunogen: Human APP-BP1

Cross-Reactivity: Mouse (Murine), Dog (Canine), Rat (Rattus)

Format: Liquid

Isotype: IgG2a

Clone: 20

Description: Amyloid precursor protein (APP) gene encodes multiple APPs ranging from 695 to 770 amino acids. These proteins are processed into beta-Amyloid peptides (39-43 amino acids), which form abnormal plaques in the cerebral cortex and blood vessel walls during Alzheimer’s disease. The transmembrane form of APP is a putative cell surface receptor that possesses neurite-promoting activity, co-localizes with the plaques found in Alzheimer’s disease, and is involved in synaptic vesicle recycling. APP binding protein 1 (APP-BP1) interacts with the C-terminus of APP, and is a relative of the ubiquitin-activating enzymes (E1). The structure of APP-BP1 includes a human Uba3 binding site (UBS) at amino acids 443 to 479. This site may be important for APP-BP1 regulation of the cell cycle through interactions with ubiquitinylation-related pathways. Transfection of APP-BP1 in cells with a ts41 mutation suppresses the abnormal S-phases observed in these cells in a hUba3- and hUbc12-dependent manner. In addition, APP-BP1 has been implicated in ubiquitinylation-dependent apoptosis in neurons. Thus, APP-BP1 may be a multi-functional APP-binding protein that regulates cell cycle dynamics.
Synonyms: Amyloid Precursor Protein-Binding Protein 1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 59 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of APP-BP1 on a RSV-3T3 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- APP-BP1 antibody.

Publications

Product: Chow, Korenberg, Chen et al.: "APP-BP1, a novel protein that binds to the carboxyl-terminal region of the amyloid precursor protein." in: The Journal of biological chemistry, Vol. 271, Issue 19, pp. 11339-46, 1996 (PubMed).

Hori, Osaka, Chiba et al.: "Covalent modification of all members of human cullin family proteins by NEDD8." in: Oncogene, Vol. 18, Issue 48, pp. 6829-34, 2000 (PubMed).

Chen, McPhie, Hirschberg et al.: "The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons." in: The Journal of biological chemistry, Vol. 275, Issue 12, pp. 8929-35, 2000 (PubMed).