Neural Cell Adhesion Molecule 1 (NCAM1) antibody

Antigen: Neural Cell Adhesion Molecule 1 (NCAM1)

Synonyms: CD56, NCAM, MSK39, ncam, fj56g09, wu:fj56g09, NCAM1, n-cam, LOC397761

Clonality: Monoclonal (1213C3-D5)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96870

Additional Information

Alternative name: CD56

Immunogen: BALB/C mice were injected with membrane preparation of a small cell lung carcinoma.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 1213C3-D5

Specificity: This antibody is specific to two proteins of 185 kD and 145 kD, identified as two isoforms of neural cell adhesion molecules (NCAM/CD56). NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Neuroblastoma. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with CD56, NCAM-1 antibody

Publications

Publications: Kibbelaar, Moolenaar, Michalides et al.: "Neural cell adhesion molecule expression, neuroendocrine differentiation and prognosis in lung carcinoma." in: European journal of cancer (Oxford, England : 1990), Vol. 27, Issue 4, pp. 431-5, 1991 (PubMed).

Michalides, Kwa, Springall et al.: "NCAM and lung cancer." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 8, pp. 34-7, 1994 (PubMed).