DOK2 (AA 314-412) antibody

Details for Product No. ABIN968705
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Antigen
Synonyms DokR, Frip, Dok-2, p56DOK, p56dok-2
Epitope
AA 314-412
(38), (27), (13), (12), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(150), (68), (49), (48), (41), (38), (24), (2)
Host
Mouse
(140), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1)
Application
Western Blotting (WB)
(103), (54), (40), (37), (35), (31), (9), (7), (3), (1)
Pubmed 3 references available
Quantity 50 μg
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Catalog No. ABIN968705
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Immunogen Mouse p56[dok2]
Clone Ghr5
Isotype IgG1
Cross-Reactivity Human, Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name p56 Dok2
Background P62dok (downstream of tyrosine kinases) was identified as a target of protein tyrosine kinases. Following phosphorylation, p62[dok] binds to Ras GTPase activatin protein (RasGap), indicating a role for p62[dok] in intracellular signaling pathways. p62[dok] contains several motifs that signify its important interactions with signaling proteins. These domains include the pleckstrin homology (PH) domain in the amino terminus, numerous tyrosines in the C-terminus, and ten PXXP motifs. p56[dok2] (Dok-R, FRIP) is 35% identical to p62[dok], and contains a PH domain, a central Dok homology motif (DKH), multiple tyrosine phosphorylation sites, and six PXXP motifs. Similar to p62[dok], p56[dok2] binds p120 RasGap, and may act as an adaptor molecule between RasGap and receptor complexes. p56[dok2] is expressed in tissues of hematopoietic origin, and is phosphorylated by the cytokines, IL-2, IL-3, and IL-4. In addition, p56[dok2] may act as an adaptor signaling molecule for the endothelial-specific receptor tyrosine kinase (RTK), Tek/Tie2. Thus, p56[dok2] is a second member of the Dok RasGap-binding family of proteins, which act as adaptor molecules in RTK signaling.
Molecular Weight 56 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-DOK2 (AA 314-412) antibody Western blot analysis of p56[dok2] on MEL cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-p56[dok2].
Product cited in: Di Cristofano, Carpino, Dunant et al.: "Molecular cloning and characterization of p56dok-2 defines a new family of RasGAP-binding proteins." in: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 4827-30, 1998 (PubMed).

Nelms, Snow, Hu-Li et al.: "FRIP, a hematopoietic cell-specific rasGAP-interacting protein phosphorylated in response to cytokine stimulation." in: Immunity, Vol. 9, Issue 1, pp. 13-24, 1998 (PubMed).

Jones, Dumont: "The Tek/Tie2 receptor signals through a novel Dok-related docking protein, Dok-R." in: Oncogene, Vol. 17, Issue 9, pp. 1097-108, 1998 (PubMed).

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